Cubillos A, Ulloa J, Cubillos V, Cook J K
Department of Avian Pathology, Universidad Austral de Chile, Valdivia, Chile.
Avian Pathol. 1991 Mar;20(1):85-99. doi: 10.1080/03079459108418744.
Nine isolates of infectious bronchitis (IB)-like viruses were made from 23 flocks (broilers or layers) in Chile experiencing the types of disease problems commonly associated with IBV. Their identity as IB viruses was confirmed. The histological changes they caused in tracheal organ cultures (OC) are described. Serum neutralisation tests performed in embryonated eggs (alpha-method) suggested that four of the isolates were serologically related to the Massachusetts (Mass) serotype of IBV and one to Connecticut. The four other strains were examined further by a serum neutralisation test in OC (ss-method). One was found to be of the Mass serotype but the others were found to be unrelated antigenically to a wide range of IBV serotypes isolated in many countries over a number of years. One of these three strains and the Mass strain, when given intranasally to 8-day-old specified pathogen free chickens together with pathogenic serotypes of E.coli, caused some mortality and considerable morbidity. The H120 vaccine strain was found not to protect completely against challenge with these four strains 21 days later.
从智利23个鸡群（肉鸡或蛋鸡）中分离出9株传染性支气管炎（IB）样病毒，这些鸡群出现了通常与传染性支气管炎病毒（IBV）相关的疾病问题。它们作为IB病毒的身份得到了确认。描述了它们在气管器官培养物（OC）中引起的组织学变化。在鸡胚中进行的血清中和试验（α法）表明，其中4株分离株与IBV的马萨诸塞州（Mass）血清型血清学相关，1株与康涅狄格州血清型相关。另外4株菌株通过在OC中进行的血清中和试验（ss法）进一步检测。发现其中1株为Mass血清型，但其他菌株在抗原性上与多年来在许多国家分离出的多种IBV血清型无关。这3株菌株中的1株和Mass菌株，与致病性大肠杆菌血清型一起经鼻内接种8日龄无特定病原体鸡后，导致了一些死亡和相当高的发病率。21天后发现H120疫苗株不能完全保护鸡免受这4株菌株的攻击。
