Ye LiBin, Zhang JingSong, Zhou Kan, Yang Yan, Zhou Shuai, Jia Wei, Hao RuiXia, Pan YingJie
College of Life Sciences, Nanjing Agricultural University, Nanjing, P. R. China.
Planta Med. 2008 Nov;74(14):1730-4. doi: 10.1055/s-2008-1081354. Epub 2008 Aug 5.
A water-soluble polysaccharide (LZ-D-1) was isolated from Ganoderma lucidum fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-300 high-resolution chromatography. The polysaccharide had a molecular weight of 2.8 x 10(4) Da and was mainly composed of L-Fuc, D-Glc and D-Gal in a molar ratio of ca.1 : 1 : 5. LZ-D-1 had a sugar content of 98.7 % as measured using the phenol-sulfuric acid method. On the basis of methylation analysis, FT-IR spectrum and NMR experiments, the structure of the repeating unit of the polysaccharide was established. LZ-D-1 had a backbone composed of 1,6-disubstituted alpha-D-galactopyranosyl, 1,2,6-trisubstituted alpha-D-galactopyranosyl and had a branch composed of fucose attached to O-2 of a 1,2,6-trisubstituted alpha-D-galactopyranosyl residue. At the same time, the polysaccharide contained some 1,6-disubstituted glucopyranosyl residues and 1,3-disubstituted glucopyranosyl residues. An immunostimulating experiment in vitro indicated that LZ-D-1 stimulated proliferation of mouse spleen lymphocytes.
通过DEAE-琼脂糖快速流动柱层析和Sephacryl S-300高分辨率层析从灵芝子实体中分离出一种水溶性多糖（LZ-D-1）。该多糖分子量为2.8×10⁴Da，主要由L-岩藻糖、D-葡萄糖和D-半乳糖组成，摩尔比约为1 : 1 : 5。采用苯酚-硫酸法测定，LZ-D-1的含糖量为98.7%。基于甲基化分析、傅里叶变换红外光谱和核磁共振实验，确定了该多糖重复单元的结构。LZ-D-1具有由1,6-二取代的α-D-吡喃半乳糖基、1,2,6-三取代的α-D-吡喃半乳糖基组成的主链，且具有一个由岩藻糖连接到1,2,6-三取代的α-D-吡喃半乳糖基残基的O-2位上构成的支链。同时，该多糖还含有一些1,6-二取代的吡喃葡萄糖基残基和1,3-二取代的吡喃葡萄糖基残基。体外免疫刺激实验表明，LZ-D-1能刺激小鼠脾淋巴细胞增殖。
