Sun Yongxu, Liang Haitao, Zhang Xiantao, Tong Haibin, Liu Jicheng
Department of Pharmacology, Qiqihar Medical University, Qiqihar, China.
Bioresour Technol. 2009 Mar;100(5):1860-3. doi: 10.1016/j.biortech.2008.09.036. Epub 2008 Nov 17.
The water-soluble polysaccharide (AMP), with a molecular mass of 7.8x10(3)Da as determined by high-performance size-exclusion chromatography (HPSEC), was obtained from the fruiting body of Armillaria mellea. Methylation, Smith degradation, acetolysis, (1)H and (13)C NMR spectroscopy and acid hydrolysis studies were conducted to elucidate its structure. The results indicated that AMP consisted of a backbone composed of (1-->6)-linked-alpha-D-glucopyranosyl, (1-->2,6)-linked-alpha-D-glucopyranosyl and (1-->6)-linked-alpha-D-galactopyranosyl residues in the ratio of 3:1:1, and terminated with one single terminal (1-->)-beta-D-glucopyranosyl at the O-2 position of (1-->2,6)-linked-alpha-D-glucopyranosyl, on average, along the main chain. Preliminary tests in vitro showed that AMP has stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide in a dose-dependent manner. It is a possible potential immunopotentiating agent for use in health-care food or medicine.
通过高效尺寸排阻色谱（HPSEC）测定，从蜜环菌子实体中获得了分子量为7.8x10(3)Da的水溶性多糖（AMP）。进行了甲基化、史密斯降解、乙酰解、（1）H和（13）C核磁共振光谱以及酸水解研究以阐明其结构。结果表明，AMP由（1→6）连接的α-D-吡喃葡萄糖基、（1→2,6）连接的α-D-吡喃葡萄糖基和（1→6）连接的α-D-吡喃半乳糖基残基组成，比例为3:1:1，并且平均沿着主链在（1→2,6）连接的α-D-吡喃葡萄糖基的O-2位置以一个单一的末端（1→）-β-D-吡喃葡萄糖基终止。体外初步试验表明，AMP对刀豆球蛋白A或脂多糖诱导的小鼠淋巴细胞增殖具有剂量依赖性刺激作用。它是一种可能用于保健食品或药品的潜在免疫增强剂。
