Bai Ru-feng, Shi Mei-sen, Yu Xiao-jun, Na Zhi-ya
China University of Political Science and Law, Ministry of Education, Beijing, 100040 People's Republic of China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Aug;25(4):469-72.
To investigate the genetic polymorphisms of 11 Y-chromosomal short tandem repeats (Y-STR) loci in 484 male individuals from two minority populations, the Hui and Xibe, of Liaoning province, and to evaluate their forensic application values and genetic relationships with other 15 populations of China.
Eleven Y-STR loci in all samples were amplified with PowerPlex Y System, and the PCR products were analyzed by 310 Genetic Analyzer. Cluster analysis and neighbor-joining tree were applied to show the genetic distance among the populations.
In Hui people, 187 haplotypes were identified, and the overall haplotype diversity value was 0.9990. The gene diversity values (GD) for each locus ranged from 0.4783(DYS437) to 0.9679(DYS385a/b); In Xibe people, 237 haplotypes were identified, and the overall haplotype diversity value was 0.9984. The GD value for each locus ranged from 0.3618(DYS391) to 0.9686(DYS385a/b). Comparing with 15 reference populations, the genetic distance between the Hui and Xibe was the nearest (0.0257), and that between the Hui and Yi was the farthest (0.1046), while the genetic distance between Xibe and Korean was also the farthest (0.0978). The NJ tree was similar to the results of clustering analysis and all the 17 populations were clustered into 3 groups.
The genetic distribution of the 11 Y-STR loci in Liaoning Hui and Xibe ethnic groups showed favorable polymorphisms, therefore are suitable for forensic identification and paternity testing in the local area. The study of haplotype diversity among different populations is useful in understanding their origins, migrations and their relationships.
研究辽宁省回族和锡伯族两个少数民族484名男性个体中11个Y染色体短串联重复序列（Y-STR）基因座的遗传多态性，评估其法医学应用价值以及与中国其他15个群体的遗传关系。
采用PowerPlex Y系统对所有样本中的11个Y-STR基因座进行扩增，PCR产物用310遗传分析仪进行分析。应用聚类分析和邻接树来显示群体间的遗传距离。
回族人群中，共鉴定出187种单倍型，总体单倍型多样性值为0.9990。各基因座的基因多样性值（GD）范围为0.4783（DYS437）至0.9679（DYS385a/b）；锡伯族人群中，共鉴定出237种单倍型，总体单倍型多样性值为0.9984。各基因座的GD值范围为0.3618（DYS391）至0.9686（DYS385a/b）。与15个参考群体比较，回族与锡伯族的遗传距离最近（0.0257），回族与彝族的遗传距离最远（0.1046），锡伯族与朝鲜族的遗传距离也最远（0.0978）。邻接树结果与聚类分析结果相似，17个群体聚为3组。
辽宁省回族和锡伯族人群11个Y-STR基因座的遗传分布呈现出良好的多态性，适用于当地的法医学个体识别和亲子鉴定。研究不同群体间的单倍型多样性有助于了解其起源、迁徙及相互关系。
