Shi Mei-Sen, Bai Ru-Feng, Fu Bo
Key Laboratory of Evidence Science, Ministry of Education, China University of Political Science and Law, Beijing 100088, China.
Yi Chuan. 2011 Mar;33(3):228-38. doi: 10.3724/sp.j.1005.2011.00228.
To investigate the genetic polymorphisms of 17 Y-chromosomal short tandem repeats (Y-STR) loci in Shanxi Han population and to explore their forensic application values and genetic relationship with neighboring populations, 17 Y-STR in 222 unrelated healthy Shanxi Han individuals were amplified with Y-filerTM System, and the PCR products were detected with 3130 Genetic Aanalyzer. The allele frequencies and haplotype diversity were calculated. Y-STR data of another 13 populations were collected from publications. Cluster analysis and phylogenic trees were applied to show the genetic distance among the populations. As a result, a total of 219 haplotypes were identified, and the observed haplotypes diversity value was 0.9999. The gene diversity values (GD) for each locus ranged from 0.3894 (DYS391) to 0.9755 (DYS385a/b). Comparing with 13 populations, the genetic distance between Shanxi and Jihei Han populations was the smallest (0.0001), while the genetic distance between Shanxi and Taiwan population was the largest (0.0152). The phylogenetic tree was similar to the results of clustering analysis. Multiplex detection of the 17 Y-STR loci revealed a highly polymorphic genetic distribution, which would be very powerful for establishing a Y-STR database, for population genetics and forensic practice.
为研究山西汉族人群17个Y染色体短串联重复序列（Y-STR）基因座的遗传多态性，探讨其法医学应用价值及与邻近人群的遗传关系，采用Y-filerTM系统对222名无血缘关系的山西汉族健康个体的17个Y-STR进行扩增，PCR产物用3130遗传分析仪检测。计算等位基因频率和单倍型多样性。从文献中收集另外13个群体的Y-STR数据。应用聚类分析和系统发育树展示群体间的遗传距离。结果，共鉴定出219种单倍型，观察到的单倍型多样性值为0.9999。各基因座的基因多样性值（GD）范围为0.3894（DYS391）至0.9755（DYS385a/b）。与13个群体比较，山西汉族与冀黑汉族群体间的遗传距离最小（0.0001），而山西汉族与台湾群体间的遗传距离最大（0.0152）。系统发育树与聚类分析结果相似。17个Y-STR基因座的多重检测显示出高度多态的遗传分布，这对建立Y-STR数据库、群体遗传学研究和法医学实践具有重要意义。
