Cheng Shao-Ting, Chen Zhi-Fei, Chen Guo-Qiang
Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong, China.
Biomaterials. 2008 Nov;29(31):4187-94. doi: 10.1016/j.biomaterials.2008.07.022. Epub 2008 Aug 6.
In order to evaluate the possibility for making artificial blood vessels, a series of microbial copolyesters poly(3-hydroxybutyrate-co-4-hydroxybutyrate)s (P3HB4HB)s containing 0-40 mol% 4-hydroxybutyrate (4HB) were studied for growth and formation of elastin of rabbit blood vessel smooth muscle cells (RaSMCs) incubated on the solvent-casting polyester films. Porous scaffolds of all P3HB4HBs except P(HB-40 mol% 4HB) were used to compare their potentials as materials for tissue engineering blood vessel (TEBV). The polyesters were studied using static contact angles, differential scanning calorimetry (DSC), cell count kit-8 (CCK-8) and Fastin elastin assays. Simultaneously, SEM, H&E and DAPI staining were employed to study cell morphology and cell growth in the polyester scaffolds. Viability of rabbit blood vessel smooth muscle cells (RaSMCs) grown on P(HB-7 mol% 4HB) films was the strongest among all other tested polyester films during the whole growth process. H&E and DAPI staining clearly suggested good cells' growth and even confluent growth in the P3HB4HB scaffolds. Fastin elastin assay demonstrated that 4HB component in the P3HB4HB copolymer benefited the elastin formation and accumulation. P(HB-20 mol% 4HB) showed a 160% more elastin production compared with that on the well-studied poly(3-hydroxybutyrate-co-12 mol% 3-hydroxyhexanoate) (PHBHHx) incubated under the same conditions. Since the P3HB4HB has adjustable elasticity and strength, combined with its ability to induce elastin formation, it can be regarded as a useful material for developing into a material for artificial blood vessels.
为了评估制造人造血管的可能性，研究了一系列含有0-40摩尔% 4-羟基丁酸（4HB）的微生物共聚酯聚（3-羟基丁酸酯-co-4-羟基丁酸酯）（P3HB4HB），用于在溶剂浇铸聚酯薄膜上培养的兔血管平滑肌细胞（RaSMCs）的生长和弹性蛋白形成。除了P（HB-40摩尔% 4HB）之外的所有P3HB4HB的多孔支架被用于比较它们作为组织工程血管（TEBV）材料的潜力。使用静态接触角、差示扫描量热法（DSC）、细胞计数试剂盒-8（CCK-8）和Fastin弹性蛋白测定法对这些聚酯进行了研究。同时，采用扫描电子显微镜（SEM）、苏木精-伊红（H&E）染色和4',6-二脒基-2-苯基吲哚（DAPI）染色来研究聚酯支架中的细胞形态和细胞生长。在整个生长过程中，在P（HB-7摩尔% 4HB）薄膜上生长的兔血管平滑肌细胞（RaSMCs）的活力在所有其他测试的聚酯薄膜中最强。H&E和DAPI染色清楚地表明在P3HB4HB支架中有良好的细胞生长和均匀的汇合生长。Fastin弹性蛋白测定表明，P3HB4HB共聚物中的4HB成分有利于弹性蛋白的形成和积累。与在相同条件下培养的研究充分的聚（3-羟基丁酸酯-co-12摩尔% 3-羟基己酸酯）（PHBHHx）相比，P（HB-20摩尔% 4HB）的弹性蛋白产量高出160%。由于P3HB4HB具有可调节的弹性和强度，结合其诱导弹性蛋白形成的能力，它可被视为一种有用于开发成人造血管材料的有用材料。
