Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, China.
Biomaterials. 2010 May;31(14):3967-75. doi: 10.1016/j.biomaterials.2010.01.132. Epub 2010 Feb 12.
Polyhydroxyalkanoates (PHA) have demonstrated their potentials as medical implant biomaterials. Neural stem cells (NSCs) grown on/in PHA scaffolds may be useful for repairing central nervous system (CNS) injury. To investigate this possibility, nanofiber matrices (scaffolds) prepared from several PHA via a novel phase separation process were studied to mimic natural extracellular matrix (ECM), and rat-derived NSCs grown in the PHA matrices were characterized regarding their in vitro differentiation behaviors. All three PHA materials including poly(3-hydroxybutyrate) (PHB), copolymer of 3-hydroxybutyrate and 4-hydroxybutyrate (P3HB4HB), and copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) supported NSC growth and differentiation both on their 2D films and 3D matrices. Among three PHA nanofiber matrices, PHBHHx one showed the strongest potentials to promote NSC differentiation into neurons which is beneficial for CNS repair. Compared to the 2D films, 3D nanofiber matrices appeared to be more suitable for NSC attachment, synaptic outgrowth and synaptogenesis. It was suggested that PHBHHx nanofiber scaffolds (matrices) that promote NSC growth and differentiation, can be developed for treating central nervous system injury.
聚羟基烷酸酯（PHA）已被证明具有作为医学植入生物材料的潜力。在 PHA 支架上生长的神经干细胞（NSC）可能有助于修复中枢神经系统（CNS）损伤。为了研究这种可能性，通过一种新的相分离工艺制备了几种 PHA 的纳米纤维基质（支架），以模拟天然细胞外基质（ECM），并对在 PHA 基质中生长的大鼠来源的 NSC 进行了体外分化行为的特征分析。包括聚（3-羟基丁酸酯）（PHB）、3-羟基丁酸酯和 4-羟基丁酸酯共聚物（P3HB4HB）以及 3-羟基丁酸酯和 3-羟基己酸酯共聚物（PHBHHx）在内的三种 PHA 材料均支持 NSC 在其 2D 薄膜和 3D 基质上的生长和分化。在三种 PHA 纳米纤维基质中，PHBHHx 对促进 NSC 分化为神经元的潜力最强，这有利于 CNS 修复。与 2D 薄膜相比，3D 纳米纤维基质似乎更适合 NSC 附着、突触生长和突触形成。因此，可开发促进 NSC 生长和分化的 PHBHHx 纳米纤维支架（基质）来治疗中枢神经系统损伤。
