Li Jian, Zhang Xu, Udduttula Anjaneyulu, Fan Zhi Shan, Chen Jian Hai, Sun Antonia RuJia, Zhang Peng
Shenzhen Engineering Research Center for Medical Bioactive Materials, Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2021 Dec 21;9:763031. doi: 10.3389/fbioe.2021.763031. eCollection 2021.
Polyhydroxyalkanoates (PHAs) are a class of structurally diverse natural biopolyesters, synthesized by various microbes under unbalanced culture conditions. PHAs as biomedical materials have been fabricated in various forms to apply to tissue engineering for the past years due to their excellent biodegradability, inherent biocompatibility, modifiable mechanical properties, and thermo-processability. However, there remain some bottlenecks in terms of PHA production on a large scale, the purification process, mechanical properties, and biodegradability of PHA, which need to be further resolved. Therefore, scientists are making great efforts synthetic biology and metabolic engineering tools to improve the properties and the product yields of PHA at a lower cost for the development of various PHA-based scaffold fabrication technologies to widen biomedical applications, especially in bone tissue engineering. This review aims to outline the biosynthesis, structures, properties, and the bone tissue engineering applications of PHA scaffolds with different manufacturing technologies. The latest advances will provide an insight into future outlooks in PHA-based scaffolds for bone tissue engineering.
聚羟基脂肪酸酯（PHA）是一类结构多样的天然生物聚酯，由各种微生物在不平衡的培养条件下合成。由于其优异的生物降解性、固有的生物相容性、可调节的机械性能和热加工性，在过去几年中，PHA作为生物医学材料已被制成各种形式应用于组织工程。然而，在PHA的大规模生产、纯化过程、机械性能和生物降解性方面仍存在一些瓶颈，需要进一步解决。因此，科学家们正在大力运用合成生物学和代谢工程工具，以较低的成本改善PHA的性能和产品产量，开发各种基于PHA的支架制造技术，以拓宽生物医学应用，特别是在骨组织工程方面。本综述旨在概述采用不同制造技术的PHA支架的生物合成、结构、性能及其在骨组织工程中的应用。最新进展将为基于PHA的骨组织工程支架的未来展望提供见解。
