Takeyama H, Wakamiya N, O'Hara C, Arthur K, Niloff J, Kufe D, Sakarai K, Spriggs D
Laboratory of Clinical Pharmacology, Dana Farber Cancer Institute, Boston, Massachusetts 02115.
Cancer Res. 1991 Aug 15;51(16):4476-80.
Tumor necrosis factor (TNF) is a cytokine produced by monocytes and other cells with selective cytolytic activity against some but not all tumor cells. Cellular resistance to the cytolytic effects of TNF has been reported to be associated with autocrine production of TNF by the target cells. The purpose of this study was to determine whether or not human tumors produce tumor necrosis factor in vivo. Ovarian carcinoma tissue from 25 patients with ovarian carcinoma was examined for the presence of TNF. Four of 5 ascites fluid specimens and tissue sections of 16 of 20 patients were positive for TNF by immunoperoxidase staining. The source of the immunoreactive protein was further examined by in situ hybridization studies. TNF mRNA was detectable in each of the ascites specimens and 7 of 16 tissue sections that were positive by immunoperoxidase staining. These findings suggest that TNF is produced by some human tumors in vivo and that the association between TNF production and resistance to TNF antitumor action may be clinically relevant.
肿瘤坏死因子（TNF）是一种由单核细胞和其他细胞产生的细胞因子，对部分而非全部肿瘤细胞具有选择性细胞溶解活性。据报道，细胞对TNF细胞溶解作用的抗性与靶细胞自分泌TNF有关。本研究的目的是确定人类肿瘤在体内是否产生肿瘤坏死因子。对25例卵巢癌患者的卵巢癌组织进行了TNF检测。通过免疫过氧化物酶染色，5份腹水样本中的4份以及20例患者中16例的组织切片TNF呈阳性。通过原位杂交研究进一步检测了免疫反应性蛋白的来源。在每份腹水样本以及免疫过氧化物酶染色呈阳性的16份组织切片中的7份中可检测到TNF mRNA。这些发现表明，TNF由一些人类肿瘤在体内产生，并且TNF产生与对TNF抗肿瘤作用的抗性之间的关联可能具有临床相关性。
