Harburger Lauren L, Pechenino Angela S, Saadi Altaf, Frick Karyn M
Department of Psychology, 2 Hillhouse Avenue, Yale University, New Haven, CT 06520, USA.
Behav Brain Res. 2008 Dec 12;194(2):174-80. doi: 10.1016/j.bbr.2008.07.014. Epub 2008 Jul 18.
The aim of this study was to determine if progesterone modulates object and spatial memory consolidation in young ovariectomized C57BL/6 mice. Object memory was tested in an object recognition task using 24- and 48-h delays. Spatial memory was tested in a 2-day version of the Morris water maze in which retention was tested 24 or 48 h after training. Immediately after training in each task, mice received a single intraperitoneal injection of vehicle or 5, 10, or 20mg/kg water-soluble progesterone. Mice were then tested 24 or 48 h later in the absence of circulating progesterone. Post-training injections of 10 and 20mg/kg progesterone enhanced object recognition, but not memory in the spatial water maze. These findings suggest that object memory consolidation in young female mice is more sensitive to the modulatory effects of progesterone than spatial memory consolidation, at least using the tasks, doses, and delays tested. As such, these findings may have important implications for the design of progesterone therapies intended to reduce age-related memory decline.
本研究的目的是确定孕酮是否调节年轻去卵巢C57BL/6小鼠的物体和空间记忆巩固。在物体识别任务中，使用24小时和48小时延迟来测试物体记忆。在莫里斯水迷宫的2天版本中测试空间记忆，其中在训练后24小时或48小时测试记忆保持情况。在每个任务训练后，小鼠立即接受单次腹腔注射溶剂或5、10或20mg/kg水溶性孕酮。然后在没有循环孕酮的情况下，于24小时或48小时后对小鼠进行测试。训练后注射10mg/kg和20mg/kg孕酮可增强物体识别能力，但对空间水迷宫中的记忆没有影响。这些发现表明，至少在所测试的任务、剂量和延迟条件下，年轻雌性小鼠的物体记忆巩固比空间记忆巩固对孕酮的调节作用更敏感。因此，这些发现可能对旨在减少与年龄相关的记忆衰退的孕酮疗法设计具有重要意义。
