Bierne H, Ehrlich S D, Michel B
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
EMBO J. 1991 Sep;10(9):2699-705. doi: 10.1002/j.1460-2075.1991.tb07814.x.
Hybrids composed of phage M13, plasmid pBR322 and the termination signal of Escherichia coli chromosome replication terB were used to show that arrest of DNA synthesis creates a very efficient deletion hot spot. Up to 80% of deletions occurring in these hybrids had one deletion end-point at terB provided that (i) terB was oriented to arrest M13 and pBR322 leading strand synthesis; and (ii) the host cells contained the Tus protein necessary for arresting DNA synthesis at terB. The position of terB and the flanking sequences had little effect on deletion hot spot activity. About 90% of the deletions at terB ended 5-6 nucleotides in front of the major replication arrest site. We propose two models to account for deletion formation and speculate that many genome rearrangements may be due to the pausing of DNA replication.
由噬菌体M13、质粒pBR322和大肠杆菌染色体复制终止信号terB组成的杂种用于表明DNA合成的停滞产生了一个非常有效的缺失热点。只要（i）terB的方向是阻止M13和pBR322前导链的合成；以及（ii）宿主细胞含有在terB处阻止DNA合成所需的Tus蛋白，这些杂种中发生的缺失有高达80%在terB处有一个缺失端点。terB的位置和侧翼序列对缺失热点活性影响很小。terB处约90%的缺失在主要复制停滞位点前5-6个核苷酸处结束。我们提出了两个模型来解释缺失的形成，并推测许多基因组重排可能是由于DNA复制的暂停。
