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高速逆流色谱法分离鉴定山荆子果实花色苷成分及其抗氧化活性评价

Isolation and Identification of Anthocyanin Component in the Fruits of (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity.

机构信息

College of Bioengineering, Beijing Polytechnic, Beijing 100176, China.

MSD R&D (China) Ltd. Co., Beijing, 100029, China.

出版信息

Molecules. 2020 Apr 13;25(8):1781. doi: 10.3390/molecules25081781.

DOI:10.3390/molecules25081781
PMID:32295006
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7221754/
Abstract

(Rupr. & Maxim.) Seem. () is one of the most abundant species of genus . The fruits of are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of . The anthocyanin content in the fresh fruits of was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), H- and C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

摘要

（Rupr. & Maxim.）Seem.（）是属中最丰富的物种之一。的果实被用于传统医学方案中，作为一种镇痛药、滋补剂、抗糖尿病药、抗高血压药、抗炎药、抗肿瘤药和免疫刺激剂。在这项工作中，我们对进行了全面调查。采用高效液相色谱-二极管阵列检测（HPLC/DAD）法测定新鲜果实中的花色苷含量，采用高速逆流色谱（HSCCC）法从这些果实中分离花色苷成分，并通过电喷雾电离质谱（ESI/MS）、H-和 C-NMR 对其进行阐明。采用铁还原抗氧化能力（FRAP）和 2,2-二苯基-1-苦基肼（DPPH）法评价其抗氧化活性。我们发现含有 121.35 毫克/100 克的总花色苷含量。HSCCC 成功地用于分离和纯化主要的花色苷成分，矢车菊素 3-木糖基半乳糖苷。抗氧化和自由基清除试验表明，矢车菊素 3-木糖基半乳糖苷是一种有效的抗氧化剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/b433304fe52c/molecules-25-01781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/4c423a199a2f/molecules-25-01781-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/99a1e14dfd26/molecules-25-01781-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/a0940cd919d8/molecules-25-01781-g0A3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/7e7da61ba37f/molecules-25-01781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/188e45bc726f/molecules-25-01781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/a6ed3c2a711b/molecules-25-01781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/562a6c2870cd/molecules-25-01781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/334f6ec277ca/molecules-25-01781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/0b7fda09d5ed/molecules-25-01781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/b433304fe52c/molecules-25-01781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/4c423a199a2f/molecules-25-01781-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/99a1e14dfd26/molecules-25-01781-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/a0940cd919d8/molecules-25-01781-g0A3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/7e7da61ba37f/molecules-25-01781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/188e45bc726f/molecules-25-01781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/a6ed3c2a711b/molecules-25-01781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/562a6c2870cd/molecules-25-01781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/334f6ec277ca/molecules-25-01781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/0b7fda09d5ed/molecules-25-01781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8d0/7221754/b433304fe52c/molecules-25-01781-g007.jpg

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