Hu Xiaojia, Jin Huizi, Xu Wenzheng, Zhang Wei, Liu Xiaohua, Yan Shikai, Chen Ming, Li Jianqiang, Zhang Wei-dong
School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, PR China.
J Ethnopharmacol. 2008 Oct 30;120(1):118-22. doi: 10.1016/j.jep.2008.07.010. Epub 2008 Jul 18.
Daphne retusa Hemsl. belongs to the genus Daphne, a member of Thymelaeaceae family. The barks and stems of Daphne retusa are used as a folkloric medicine 'Zhu Shi Ma' in Western China because of its effects of detumescence and acesodyne. In this paper, we investigate the anti-inflammatory and analgesic effects of the 75% ethanol extract of the stems and barks of Daphne retusa and different fractions partitioned with petroleum ether, methylene chloride, ethyl acetate and n-butanol, respectively. The anti-inflammatory effects were evaluated using xylene-induced ear oedema in mice and carrageenan-induced paw oedema in rats, while the acetic acid-induced writhing test and hot-plate test as models for evaluating the centrally and peripherally analgesic activity. The results showed the plant has significant anti-inflammatory and analgesic effects (P<0.05-0.01). Meanwhile, the result of the acute toxicity test at which the MTD was above 5g/kg indicates that the plant extract is relatively safe in, and/or non-toxic to, mice. The findings of these experimental animal studies indicate that the Daphne retusa ethanol extract possesses anti-inflammatory and analgesic properties, and thus provide pharmacological support to folkloric, ethnomedical uses of 'Zhu shima' in the treatment and/of management of anti-inflammatory and painful conditions in China.
小黄构属于瑞香属，是瑞香科的一员。小黄构的树皮和茎在中国西部被用作民间药物“猪屎麻”，因其具有消肿止痛的功效。在本文中，我们研究了小黄构茎皮75%乙醇提取物以及分别用石油醚、二氯甲烷、乙酸乙酯和正丁醇分离得到的不同萃取物的抗炎和镇痛作用。采用二甲苯致小鼠耳肿胀和角叉菜胶致大鼠足肿胀模型评价其抗炎作用，以醋酸致小鼠扭体试验和热板试验作为评价中枢和外周镇痛活性的模型。结果表明该植物具有显著的抗炎和镇痛作用（P<0.05 - 0.01）。同时，最大耐受量高于5g/kg的急性毒性试验结果表明该植物提取物对小鼠相对安全和/或无毒。这些实验动物研究结果表明小黄构乙醇提取物具有抗炎和镇痛特性，从而为“猪屎麻”在中国用于治疗和管理炎症及疼痛性疾病的民间医学应用提供了药理学支持。
