Shi L-Y, Li M, Yuan L-J, Wang Q, Li X-M
Department of Microbiology, The College of Basic Medical Science, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, China.
Arch Virol. 2008;153(9):1715-23. doi: 10.1007/s00705-008-0184-9. Epub 2008 Aug 12.
The complete RNA genome sequence of Tianjin strain, isolated from marmoset, has been determined. The genome was 15,384 nucleotides (nt) in length, which was identical to that of Sendai virus (SeV), and consisted of six genes in the order 3'-NP-P/C-M-F-HN-L-5'. The gene junctions contained highly conserved transcription start and stop signal sequences and trinucleotide intergenic regions (IGR) similar to those of SeV. There was also an overlapping open reading frame and a conserved AnGn site (AAAAAGGG) in the P mRNA. Phylogenetic analysis based on protein sequences from other members of the family Paramyxoviridae showed that Tianjin strain belonged to the genus Respirovirus and was most closely related to SeV. Pairwise comparisons of amino acid identities and phylogenetic analysis with homologous sequences of known SeVs demonstrated that Tianjin strain represented a new evolutionary lineage of SeV.
已测定从狨猴分离出的天津株的完整RNA基因组序列。该基因组长度为15384个核苷酸（nt），与仙台病毒（SeV）的基因组长度相同，由六个基因组成，顺序为3'-NP-P/C-M-F-HN-L-5'。基因连接处包含高度保守的转录起始和终止信号序列以及与SeV相似的三核苷酸基因间区域（IGR）。在P mRNA中还存在一个重叠开放阅读框和一个保守的AnGn位点（AAAAAGGG）。基于副粘病毒科其他成员蛋白质序列的系统发育分析表明，天津株属于呼吸病毒属，与SeV关系最为密切。与已知SeV的同源序列进行氨基酸同一性的成对比较和系统发育分析表明，天津株代表了SeV的一个新进化谱系。
