Suresh Kumar S V, Mishra S H
Pharmacy Department, The M.S. University of Baroda, Vadodara 390 001, India.
Indian J Exp Biol. 2008 Jun;46(6):447-52.
Ethanol extract and its ethanol fraction from aerial parts of P. daemia exhibited significant hepatoprotective effect against CCl4 induced hepatotoxicity in rats. Glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline phosphatase, total bilirubin, total cholesterol, total protein and albumin in serum indicated hepatoprotective effect of the ethanol extract and its ethanol fraction. Histopathological examination of liver sections confirmed that, pre-treatment with ethanol extract and its ethanol fraction prevented hepatic damage induced by CCl4. The results were comparable with the standard hepatoprotective drug silymarin. The extract and its fraction showed no signs of toxicity up to a dose level of 2000 mg/kg. It is suggested that, the presence of flavonoids in ethanol extract and its ethanol fraction may be responsible for hepatoprotective properties. High Performance Thin Layer Chromatography profile of flavonoids of bio-active extracts was developed using quercetin-3-glucoside as a marker. Results indicate hepatoprotective properties of ethanol extract of P. daemia.
白花丹地上部分的乙醇提取物及其乙醇馏分对四氯化碳诱导的大鼠肝毒性表现出显著的肝保护作用。血清中的谷草转氨酶、谷丙转氨酶、碱性磷酸酶、总胆红素、总胆固醇、总蛋白和白蛋白表明了乙醇提取物及其乙醇馏分的肝保护作用。肝脏切片的组织病理学检查证实，用乙醇提取物及其乙醇馏分进行预处理可预防四氯化碳诱导的肝损伤。结果与标准肝保护药物水飞蓟素相当。提取物及其馏分在剂量水平达2000mg/kg时未表现出毒性迹象。有人认为，乙醇提取物及其乙醇馏分中黄酮类化合物的存在可能是其具有肝保护特性的原因。以槲皮素-3-葡萄糖苷为标记物，建立了生物活性提取物中黄酮类化合物的高效薄层色谱图谱。结果表明白花丹乙醇提取物具有肝保护特性。
