Seemayer N, Manojlovic N, Brockhaus A
Zentralbl Bakteriol Orig B. 1976 Jul;162(1-2):27-33.
Airborne dust is toxic for cells cultured in vitro and able to transform these cells to cancerous. The effect of extracts from atmospheric dust has been investigated. The dust samples were extracted by means of DMSO alone or in combination acetone-DMSO. Dosage of the extract was done according to its benzo(a)pyrene content (mug/ml medium). Dust extract with a concentration of 1 mug benzo(a)pyrene/ml exerted a toxic effect upon mouse macrophages (cell line IC-21) and human lymphocytes after stimulation. The degree of toxicity was estimated from the percentage of damaged cells seen in the dye exclusion test and from the amount of lactate dehydrogenase and lactate released into the medium in the case of macrophages. In the case of lymphocytes the degree of toxicity was estimated from the extent of inhibition of DNA synthesis. In the carcinogenicity test, hamster kidney cells were first treated with the extract and then incubated with Simian Virus (SV-)40. Treatment of the cell cultures with extract from airborne dust in a concentration of 0.01 and 0.1 mug benzo(a)pyrene/ml clearly enhances the rate of transformation caused by SV 40.
空气中的灰尘对体外培养的细胞有毒性，并且能够将这些细胞转化为癌细胞。已对大气灰尘提取物的作用进行了研究。灰尘样本通过单独使用二甲基亚砜（DMSO）或丙酮 - 二甲基亚砜组合进行提取。提取物的剂量根据其苯并(a)芘含量（微克/毫升培养基）确定。浓度为1微克苯并(a)芘/毫升的灰尘提取物在刺激后对小鼠巨噬细胞（细胞系IC - 21）和人类淋巴细胞产生毒性作用。毒性程度通过染料排除试验中受损细胞的百分比以及巨噬细胞情况下释放到培养基中的乳酸脱氢酶和乳酸量来估计。对于淋巴细胞，毒性程度根据DNA合成的抑制程度来估计。在致癌性试验中，首先用提取物处理仓鼠肾细胞，然后与猿猴病毒（SV -）40一起孵育。用浓度为0.01和0.1微克苯并(a)芘/毫升的空气中灰尘提取物处理细胞培养物明显提高了由SV 40引起的转化速率。
