Matsubara Kiminori, Kayashima Tomoko, Mori Masaharu, Yoshida Hiromi, Mizushina Yoshiyuki
Department of Human Life Sciences Education, Graduate School of Education, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8524, Japan.
Int J Mol Med. 2008 Sep;22(3):381-7.
Vitamins play essential roles in cellular reactions and maintain human health. Recent studies have revealed that some vitamins including D3, B6 and K2 and their derivatives have an anti-cancer effect. As a mechanism, their inhibitory effect on cancer-related angiogenesis has been demonstrated. Vitamin K2 (menaquinones) has an anti-cancer effect in particular for hepatic cancer and inhibits angiogenesis. In the current study, we demonstrated that sole vitamin K3 (menadione) selectively inhibits the in vitro activity of eukaryotic DNA polymerase gamma, which is a mitochondrial DNA polymerase, and suppresses angiogenesis in a rat aortic ring model. The anti-angiogenic effect of vitamin K3 has been shown in angiogenesis models using human umbilical vein endothelial cells (HUVECs) with regard to HUVEC growth, tube formation on reconstituted basement membrane and chemotaxis. These results suggest that vitamin K3 may be a potential anti-cancer agent like vitamin K2.
维生素在细胞反应中发挥着重要作用，并维持人体健康。最近的研究表明，包括D3、B6和K2及其衍生物在内的一些维生素具有抗癌作用。作为一种机制，它们对癌症相关血管生成的抑制作用已得到证实。维生素K2（甲萘醌）尤其对肝癌具有抗癌作用，并能抑制血管生成。在本研究中，我们证明，单独的维生素K3（甲萘醌）可选择性抑制真核生物DNA聚合酶γ（一种线粒体DNA聚合酶）的体外活性，并在大鼠主动脉环模型中抑制血管生成。关于人脐静脉内皮细胞（HUVEC）的生长、在重组基底膜上形成管腔以及趋化性，维生素K3在使用HUVEC的血管生成模型中已显示出抗血管生成作用。这些结果表明，维生素K3可能是一种像维生素K2一样的潜在抗癌药物。
