Matsubara Kiminori, Mori Masaharu, Mizushina Yoshiyuki
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Oncol Rep. 2004 Feb;11(2):447-51.
Petasiphenol, a polyphenolic compound from a Japanese vegetable (Petasites japonicus) which is a DNA polymerase lambda selective inhibitor, was demonstrated as a potent antiangiogenic agent in this study. Petasiphenol showed suppressive effects on in vitro angiogenesis assays, human umbilical vein endothelial cell (HUVEC) proliferation, tube formation on reconstituted basement membrane and chemotaxis in a dose-dependent manner (10-100 microM). However, treatment of HUVEC with petasiphenol did not affect 72 kDa matrix metalloprotease activity at these concentrations. Petasiphenol at higher 50 microM also suppressed microvessel outgrowth in ex vivo angiogenesis assay using a rat aortic ring. Taken together, petasiphenol could act as a potent antiangiogenic compound for preventing tumor and be useful to design therapeutic agents.
蜂斗菜酚是一种从日本蔬菜蜂斗菜中提取的多酚类化合物，它是一种DNA聚合酶λ选择性抑制剂，在本研究中被证明是一种有效的抗血管生成剂。蜂斗菜酚对体外血管生成试验、人脐静脉内皮细胞（HUVEC）增殖、重组基底膜上的管腔形成和趋化作用具有剂量依赖性（10 - 100微摩尔）的抑制作用。然而，在这些浓度下，用蜂斗菜酚处理HUVEC不会影响72 kDa基质金属蛋白酶的活性。在使用大鼠主动脉环的离体血管生成试验中，50微摩尔更高浓度的蜂斗菜酚也抑制了微血管的生长。综上所述，蜂斗菜酚可作为一种有效的抗血管生成化合物用于预防肿瘤，并且有助于设计治疗药物。
