Susevich María Laura, Marti Gerardo Aníbal, Balsalobre Agustín, Echeverría María Gabriela
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), 2 #584, (1900) La Plata, Argentina.
Cátedra de Virología, Faculty of Veterinary Sciences (UNLP), 60 y 118 (1900) La Plata, Argentina
J Insect Sci. 2015 Mar 22;15(1). doi: 10.1093/jisesa/ieu139. Print 2015.
The only virus sequenced and studied in triatomines is the Triatoma virus, from the Dicistroviridae family, which causes delayed development, reduced oviposition, and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verifying if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by Reverse Transcription (RT)-polymerase chain reaction using the VP3-VP1 subregion of open reading frame-2 as a diagnostic method; after sequencing, 11 samples were obtained from Triatoma infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared with the sequences found during 2002-2012, all obtained from the wild. We conclude that despite being an RNA virus, the different sequences show high homology.
在锥蝽中唯一被测序和研究的病毒是来自双顺反子病毒科的锥蝽病毒，它会导致受感染昆虫发育延迟、产卵减少和过早死亡。为了扩充前几年已获得的序列，并验证其基因组序列是否发生了任何变化，对来自阿根廷几个省份的68份锥蝽样本进行了分析。使用开放阅读框2的VP3-VP1亚区域作为诊断方法，通过逆转录（RT）-聚合酶链反应获得了16份阳性样本；测序后，从侵袭锥蝽中获得了11份样本。这些新序列在分析区域没有显示出显著差异，这些区域没有按物种、栖息地或地理分布进行分组。与2002年至2012年期间发现的序列相比没有差异，所有这些序列均来自野外。我们得出结论，尽管它是一种RNA病毒，但不同序列显示出高度同源性。
