Schlatter Daniel, Fubuh Alfred, Xiao Kun, Hernandez Dan, Hobbie Sarah, Kinkel Linda
Deparment of Plant Pathology, University of Minnesota, St Paul, MN 55108, USA.
Microb Ecol. 2009 Apr;57(3):413-20. doi: 10.1007/s00248-008-9433-4. Epub 2008 Aug 13.
Carbon from plant rhizospheres is a source of energy for soil microbial communities in native habitats. Soil amendments have been used as a means for deliberately altering soil community composition in agricultural soils to enhance plant health. However, little information is available in agricultural or natural soils on how specific carbon compounds or quantities influence soil microbial communities. Streptomyces are important soil saprophytes noted for their ability to produce antibiotics and influence plant health. To explore how specific types and amounts of carbon compounds influence Streptomyces in soil, glucose, cellulose, and lignin were added alone and in combination with six other carbon substrates of varying complexity to mesocosms of native prairie soil for 9 months at amounts equivalent to natural inputs from plants. Estimated culturable population densities, antibiotic inhibitory phenotypes, and resource utilization profiles were examined for Streptomyces communities from each treatment. The type and quantity of carbon compounds influenced densities, proportions, antibiotic phenotypes, and substrate utilization profiles of Streptomyces. Cellulose and lignin inputs produced the largest Streptomyces densities. Also, Streptomyces communities receiving high-resource inputs were more inhibitory whereas those receiving low-resource inputs used substrates more efficiently. Knowledge of how the availability and quantity of particular carbon compounds influences Streptomyces communities and their function, specifically resource use and inhibitory phenotypes, may be helpful in understanding the roles of resource availability in Streptomyces community dynamics and the potential of Streptomyces to suppress pathogens and enhance plant fitness in native and agricultural soils.
植物根际的碳是原生栖息地土壤微生物群落的能量来源。土壤改良剂已被用作一种手段，有意改变农业土壤中的土壤群落组成，以促进植物健康。然而，关于特定碳化合物或数量如何影响农业土壤或天然土壤中的土壤微生物群落，目前所知甚少。链霉菌是重要的土壤腐生菌，以其产生抗生素和影响植物健康的能力而闻名。为了探究特定类型和数量的碳化合物如何影响土壤中的链霉菌，将葡萄糖、纤维素和木质素单独添加，并与其他六种不同复杂程度的碳底物组合，以相当于植物自然输入量的量添加到原生草原土壤的中型生态系统中，持续9个月。对每种处理的链霉菌群落的可培养种群密度、抗生素抑制表型和资源利用概况进行了检测。碳化合物的类型和数量影响了链霉菌的密度、比例、抗生素表型和底物利用概况。纤维素和木质素的输入产生了最大的链霉菌密度。此外，接受高资源输入的链霉菌群落抑制性更强，而接受低资源输入的群落则更有效地利用底物。了解特定碳化合物的可用性和数量如何影响链霉菌群落及其功能，特别是资源利用和抑制表型，可能有助于理解资源可用性在链霉菌群落动态中的作用，以及链霉菌在原生土壤和农业土壤中抑制病原体和提高植物适应性的潜力。
