Chojnowska Sylwia, Zalewska Anna, Knaś Małgorzata, Waszkiewicz Napoleon, Waszkiel Danuta, Kossakowska Agnieszka, Zwierz Krzysztof
Medical Institute, College of Computer Science and Business Administration, Lomza, Poland.
Department of Conservative Dentistry, Medical University in Bialystok, Poland.
Acta Biochim Pol. 2014;61(1):85-90. Epub 2014 Mar 18.
Currently we observe a growing interest in human saliva as a non-invasive material for diagnosis and monitoring of general and oral diseases.
The aim of our study was adaptation of the Marciniak et al. (Marciniak J, Zalewska A, Popko J, Zwierz K, 2006, Clin Chem Lab Med 44: 933-937) method for determination of HEX and GLU activity in synovial fluid, and for determination of: HEX and GLU, as well as MAN, GAL, and FUC activity in human saliva.
Under optimal conditions, 10 μl of saliva for HEX, and 30 μl for GLU, MAN, GAL and FUC, were sufficient for determination of human salivary exoglycosidases activity with variation coefficient ranging from 0.89 for GLU to 0.99 for GAL.
The adapted method for exoglycosidases activity determination in human saliva is sufficiently sensitive and precise to use in clinical diagnosis.
目前，我们观察到人们对人类唾液作为一种用于诊断和监测全身及口腔疾病的非侵入性材料的兴趣日益浓厚。
我们研究的目的是对Marciniak等人（Marciniak J、Zalewska A、Popko J、Zwierz K，2006年，《临床化学与实验室医学》44：933-937）的方法进行调整，以测定滑液中的己糖胺酶（HEX）和葡萄糖苷酶（GLU）活性，以及人类唾液中的HEX、GLU、甘露糖苷酶（MAN）、半乳糖苷酶（GAL）和岩藻糖苷酶（FUC）活性。
在最佳条件下，用于测定HEX的10μl唾液以及用于测定GLU、MAN、GAL和FUC的30μl唾液，足以测定人类唾液外切糖苷酶活性，变异系数范围从GLU的0.89到GAL的0.99。
所调整的用于测定人类唾液外切糖苷酶活性的方法足够灵敏和精确，可用于临床诊断。
