a Department of Prosthodontics and Periodontology , Piracicaba Dental School, University of Campinas (UNICAMP) , Piracicaba , São Paulo , Brazil.
b Department of Physiological Science , Piracicaba Dental School, University of Campinas (UNICAMP) , Piracicaba , São Paulo , Brazil.
Biofouling. 2019 Feb;35(2):173-186. doi: 10.1080/08927014.2019.1580360. Epub 2019 Apr 2.
The chemical composition of biomaterials can drive their biological responses; therefore, this in vitro study aimed to evaluate the proteomic profile of the salivary pellicle formed on titanium (Ti) alloys containing niobium (Nb) and zirconium (Zr). The experimental groups consisted of Ti35NbxZr (x = 5 and 10 wt%) alloys, and commercially pure titanium (cpTi); titanium aluminium vanadium (Ti6Al4V) alloys were used as controls. The physical and chemical characteristics of the Ti materials were analysed. The proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry. Bacterial adhesion (2 h) of mixed species (Streptococcus sanguinis and Actinomyces naeslundii) was investigated as colony-forming units (n = 6). This paper reports the finding that salivary pellicle composition can be modulated by the composition of the Ti material. The Ti35NbxZr group showed a significant ability to adsorb proteins from saliva, which can favour interactions with cells and compatibility with the body.
生物材料的化学成分可以驱动其生物反应；因此，这项体外研究旨在评估含铌 (Nb) 和锆 (Zr) 的钛 (Ti) 合金表面形成的唾液膜的蛋白质组特征。实验组由 Ti35NbxZr（x = 5 和 10wt%）合金和商用纯钛（cpTi）组成；钛铝钒（Ti6Al4V）合金用作对照。分析了 Ti 材料的物理和化学特性。通过液相色谱-串联质谱联用技术评估蛋白质组特征。以混合菌种（血链球菌和内氏放线菌）作为研究对象，通过菌落形成单位（n = 6）来研究 2 小时内的细菌黏附情况。本研究报告称，唾液膜的组成可以通过 Ti 材料的组成来调节。Ti35NbxZr 组具有从唾液中吸附蛋白质的显著能力，这有利于与细胞相互作用以及与人体的相容性。
