Qi Qi, Yao Yitong, Jia Xiaoshi, Meng Yuezhong, Zhao Ke, Jian Yutao
Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Engineering Research Center of Technology and Materials for Oral Reconstruction, Guangdong Provincial Key Laboratory of Stomatology Guangzhou China
The Key Laboratory of Low-Carbon Chemistry & Energy Conservation of Guangdong Province/State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University Guangzhou China
RSC Adv. 2019 Oct 22;9(58):33941-33948. doi: 10.1039/c9ra06654c. eCollection 2019 Oct 18.
To study the effects of polyethylene glycol (PEG) content on the mechanical properties and degradation of silk fibroin, nano-hydroxyapatite, and PEG (SF/nHAP/PEG) electrospun scaffolds, and according to the PEG ratio in the scaffold (SF : nHAP : PEG), test groups were divided as follows: PEG-0 (10 : 2), PEG-0.5 (10 : 2 : 0.5), PEG-1 (10 : 2 : 1), and PEG-2 (10 : 2 : 2). A series of tests to determine the mechanical properties, degradation rates, and osteogenic characteristics was undertaken. PEG facilitated SF degradation (PEG-1 > PEG-0.5 > PEG-0 > PEG-2), and the mass loss of the scaffolds in PEG-1 was more than 30%, while in PEG-2 it was less than 20% after 8 days ( < 0.05). The addition of PEG strengthened the mechanical properties of the scaffold (PEG-1 > PEG-2 > PEG-0.5 > PEG-0), as the Young's modulus increased from 41.72 ± 3.40 MPa for PEG-0 to 76.12 ± 3.73 MPa for PEG-1 ( < 0.05). PEG was favorable for the osteogenic differentiation of BMSCs (PEG-0.5 > PEG-1 > PEG-2 > PEG-0). The enhancements were attributable to the increased hydrophilicity and nHAP dispersion, as well as to the secondary structure transformation of SF. The PEG content was deemed to be optimal when the SF/nHAP/PEG ratio was equal to 10 : 2 : 1.
为研究聚乙二醇（PEG）含量对丝素蛋白、纳米羟基磷灰石和PEG（SF/nHAP/PEG）电纺支架的力学性能及降解的影响，根据支架中PEG的比例（SF : nHAP : PEG），将试验组分为以下几组：PEG-0（10 : 2）、PEG-0.5（10 : 2 : 0.5）、PEG-1（10 : 2 : 1）和PEG-2（10 : 2 : 2）。进行了一系列测试以确定力学性能、降解速率和成骨特性。PEG促进了SF的降解（PEG-1 > PEG-0.5 > PEG-0 > PEG-2），8天后PEG-1组支架的质量损失超过30%，而PEG-2组则小于20%（<0.05）。PEG的添加增强了支架的力学性能（PEG-1 > PEG-2 > PEG-0.5 > PEG-0），因为杨氏模量从PEG-0组的41.72±3.40 MPa增加到PEG-1组的76.12±3.73 MPa（<0.05）。PEG有利于骨髓间充质干细胞（BMSCs）的成骨分化（PEG-0.5 > PEG-1 > PEG-2 > PEG-0）。这些增强归因于亲水性和nHAP分散性的增加以及SF二级结构的转变。当SF/nHAP/PEG比例等于10 : 2 : 1时，PEG含量被认为是最佳的。
