Mohanty Suman Sundar, Raghavendra Kamaraju, Mittal Pradeep Kumar, Dash Aditya Prasad
National Institute of Malaria Research, Indian Council of Medical Research, 22-Sham Nath Marg, Delhi, India.
J Ind Microbiol Biotechnol. 2008 Oct;35(10):1199-202. doi: 10.1007/s10295-008-0434-6. Epub 2008 Aug 16.
Efficacy of culture filtrates of five strains of Metarhizium anisopliae isolated from insects were evaluated against Anopheles stephensi and Culex quinquefasciatus. The culture filtrates released from the strains of M. anisopliae in the YpSs and chitin broths were filtered and used for the bioassays after a growth of 7 days. Among the culture filtrates of five strains, M. anisopliae 892 was found to be more effective against both the mosquitoes. The LC(50) values of culture filtrates of M. anisopliae 892 in chitin broth was lower than the LC(50) of culture filtrates in YpSs broth against first and fourth instars of both the mosquitoes. The LC(50) values of culture filtrates were significantly different between first and fourth instars of A. stephensi (t test; P = 0.0001) and C. quinquefasciatus (t test; P = 0.02). The larvae of A. stephensi were more susceptible than C. quinquefasciatus except in two cases. This is the first report of efficacy of culture filtrates produced by M. anisopliae in chitin broth against mosquitoes and have potential as a biological control agent of mosquitoes.
对从昆虫中分离出的五株绿僵菌的培养滤液针对斯氏按蚊和致倦库蚊的功效进行了评估。将绿僵菌菌株在YpSs和几丁质肉汤中释放的培养滤液过滤，并在生长7天后用于生物测定。在五株菌株的培养滤液中，发现绿僵菌892对两种蚊子都更有效。绿僵菌892在几丁质肉汤中的培养滤液对两种蚊子的一龄和四龄幼虫的LC(50)值低于在YpSs肉汤中的培养滤液的LC(50)值。斯氏按蚊和致倦库蚊一龄和四龄幼虫的培养滤液LC(50)值存在显著差异（t检验；P = 0.0001和P = 0.02）。除两例情况外，斯氏按蚊幼虫比致倦库蚊幼虫更易感染。这是关于绿僵菌在几丁质肉汤中产生的培养滤液对蚊子功效的首次报道，并且有作为蚊子生物防治剂的潜力。
