Boström Elisabeth Almer, d'Elia Helena Forsblad, Dahlgren Ulf, Simark-Mattsson Charlotte, Hasséus Bengt, Carlsten Hans, Tarkowski Andrej, Bokarewa Maria
Department of Rheumatology and Inflammation Research, Section of Oral Immunology, Clinic for Oral Medicine, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
J Rheumatol. 2008 Oct;35(10):2005-11. Epub 2008 Aug 15.
To assess the role of resistin in primary Sjögren's syndrome (pSS) and its relation to local inflammation.
Blood and saliva were collected from 37 patients with pSS (duration of symptoms 12.6+/-1 yrs) and 32 healthy controls. Expression of resistin in salivary glands was visualized immunohistologically, and levels of resistin were detected by ELISA. Levels of resistin were evaluated at baseline and following oral dehydroepiandrosterone (DHEA) treatment (50 mg/day). The effect of DHEA treatment on the secretion of resistin was assessed in vitro in human leukocytes after challenge with insulin and lipopolysaccharide.
Levels of resistin in saliva were significantly higher in patients with pSS than in controls, while circulating levels of resistin were similar in both groups. Resistin was expressed in the epithelial cells of striated ducts and in the lymphocytic foci. Resistin levels in saliva were related to the intensity of inflammation in the minor salivary glands of pSS patients. No changes of the levels of resistin in blood or saliva were observed during DHEA treatment. Exposure of naive leukocytes to DHEA in vitro induced significant expression of resistin compared to nonstimulated peripheral blood mononuclear cells (p=0.031).
We showed that levels of resistin are upregulated locally in the salivary glands of patients with pSS; and that the levels of resistin correspond to the intensity of lymphocytic inflammation in patients with pSS. We suggest that resistin is expressed in the salivary glands of patients with pSS and may be a driving factor of local inflammation.
评估抵抗素在原发性干燥综合征（pSS）中的作用及其与局部炎症的关系。
收集37例pSS患者（症状持续时间12.6±1年）和32名健康对照者的血液和唾液。采用免疫组织化学方法观察抵抗素在唾液腺中的表达，并用酶联免疫吸附测定法检测抵抗素水平。在基线时以及口服脱氢表雄酮（DHEA）治疗（50毫克/天）后评估抵抗素水平。在用胰岛素和脂多糖刺激后人白细胞体外实验中评估DHEA治疗对抵抗素分泌的影响。
pSS患者唾液中抵抗素水平显著高于对照组，而两组循环中的抵抗素水平相似。抵抗素在纹状管上皮细胞和淋巴细胞灶中表达。pSS患者唾液中抵抗素水平与小唾液腺炎症强度相关。DHEA治疗期间血液或唾液中抵抗素水平未观察到变化。与未刺激的外周血单核细胞相比，体外将未接触过的白细胞暴露于DHEA可诱导抵抗素显著表达（p=0.031）。
我们发现pSS患者唾液腺中抵抗素水平局部上调；且抵抗素水平与pSS患者淋巴细胞炎症强度相关。我们认为抵抗素在pSS患者唾液腺中表达，可能是局部炎症的驱动因素。
