Nelson G N, Roh J D, Mirensky T L, Wang Y, Yi T, Tellides G, Pober J S, Shkarin P, Shapiro E M, Saltzman W M, Papademetris X, Fahmy T M, Breuer C K
Yale University School of Medicine, Interdepartmental Program in Vascular Biology and Therapeutics, Amistad Research Bldg., 10 Amistad St., Rm. 301B, P.O. Box 208089, New Haven, CT 06520, USA.
FASEB J. 2008 Nov;22(11):3888-95. doi: 10.1096/fj.08-107367. Epub 2008 Aug 18.
This pilot study examines noninvasive MR monitoring of tissue-engineered vascular grafts (TEVGs) in vivo using cells labeled with iron oxide nanoparticles. Human aortic smooth muscle cells (hASMCs) were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The labeled hASMCs, along with human aortic endothelial cells, were incorporated into eight TEVGs and were then surgically implanted as aortic interposition grafts in a C.B-17 SCID/bg mouse host. USPIO-labeled hASMCs persisted in the grafts throughout a 3 wk observation period and allowed noninvasive MR imaging of the human TEVGs for real-time, serial monitoring of hASMC retention. This study demonstrates the feasibility of applying noninvasive imaging techniques for evaluation of in vivo TEVG performance.
这项初步研究使用氧化铁纳米颗粒标记的细胞，对体内组织工程血管移植物（TEVG）进行无创磁共振监测。人主动脉平滑肌细胞（hASMC）用超小超顺磁性氧化铁（USPIO）纳米颗粒标记。将标记的hASMC与人主动脉内皮细胞一起整合到八个TEVG中，然后作为主动脉间置移植物手术植入C.B-17 SCID/bg小鼠宿主中。在整个3周的观察期内，USPIO标记的hASMC在移植物中持续存在，并允许对人TEVG进行无创磁共振成像，以实时、连续监测hASMC的留存情况。这项研究证明了应用无创成像技术评估体内TEVG性能的可行性。
