Agbandje M, McKenna R, Rossmann M G, Kajigaya S, Young N S
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Virology. 1991 Sep;184(1):170-4. doi: 10.1016/0042-6822(91)90833-w.
Crystals that diffract X rays to at least 8 A resolution have been grown from human B19 parvovirus empty capsids. These particles consist of VP-2 derived from a baculovirus expression system. This is possibly the first time that a self-assembled empty viral capsid, grown in other than normal host cells, has been crystallized. Partial X-ray diffraction data have been collected using synchrotron radiation. The space group is P2(1)3 with a = 362 A. The particle position in the crystal cell is given, at least roughly, from packing considerations.
已从人细小病毒B19空衣壳中培养出能将X射线衍射至至少8埃分辨率的晶体。这些颗粒由源自杆状病毒表达系统的VP-2组成。这可能是首次将在非正常宿主细胞中生长的自组装空病毒衣壳进行结晶。已使用同步辐射收集了部分X射线衍射数据。空间群为P2(1)3，a = 362埃。根据堆积考虑，至少大致给出了晶胞中颗粒的位置。
