犬细小病毒晶体的初步X射线晶体学分析。

Preliminary X-ray crystallographic analysis of canine parvovirus crystals.

作者信息

Luo M, Tsao J, Rossmann M G, Basak S, Compans R W

机构信息

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.

出版信息

J Mol Biol. 1988 Mar 5;200(1):209-11. doi: 10.1016/0022-2836(88)90346-4.

DOI:10.1016/0022-2836(88)90346-4

PMID:3379641

Abstract

The first diffraction pattern of a crystalline single-stranded DNA virus has been obtained. Canine parvovirus was crystallized in a monoclinic P21 unit cell with a = 264.4 A, b = 350.3 A, c = 267.8 A and beta = 90.86 degrees (1 A = 0.1 nm). The diffraction pattern extends to at least 2.8 A resolution. Packing of the particles suggests that they have a diameter around 257 A, in excellent agreement with the reported molecular weight of 5.5 x 10(6).

摘要

已获得一种结晶单链DNA病毒的首个衍射图谱。犬细小病毒在单斜P21晶胞中结晶，其a = 264.4埃，b = 350.3埃，c = 267.8埃，β = 90.86度（1埃 = 0.1纳米）。衍射图谱的分辨率至少达到2.8埃。颗粒的堆积情况表明它们的直径约为257埃，与报道的5.5×10⁶的分子量高度吻合。