Brown C S, Jensen T, Meloen R H, Puijk W, Sugamura K, Sato H, Spaan W J
Department of Virology, Faculty of Medicine, Leiden University, The Netherlands.
J Virol. 1992 Dec;66(12):6989-96. doi: 10.1128/JVI.66.12.6989-6996.1992.
An immunodominant region on baculovirus-produced parvovirus B19 VP2 capsids was localized between amino acids 259 and 426 by mapping the binding sites of a panel of monoclonal antibodies which recognize determinants on the particles. The binding sites of three monoclonal antibodies were fine-mapped within this antigenic domain. Six VP2-specific monoclonal antibodies recognized determinants common to both the empty capsids and native parvovirus. The defined antigenic region is most probably exposed on the native B19 virion and corresponds to part of the threefold spike on the surface of canine parvovirus particles.
通过绘制一组识别病毒颗粒上决定簇的单克隆抗体的结合位点，杆状病毒产生的细小病毒B19 VP2衣壳上的一个免疫显性区域定位于氨基酸259和426之间。在这个抗原结构域内对三种单克隆抗体的结合位点进行了精细定位。六种VP2特异性单克隆抗体识别空衣壳和天然细小病毒共有的决定簇。所确定的抗原区域很可能暴露在天然B19病毒体上，并且对应于犬细小病毒颗粒表面三倍体刺突的一部分。
