Dodd N J, Silcock J M
Br J Cancer. 1976 Nov;34(5):556-65. doi: 10.1038/bjc.1976.211.
Electron spin resonance (ESR) spectroscopy was used to examine changes in the concentration of paramagnetic metal ions in Yoshida tumours carried by female Wistar rats. Blood, spleen and lymph nodes from these animals were also examined by ESR. A decrease in the concentration of a paramagnetic species associated with mitochondrial activity, and marked increases in those thought to be associated with inflammatory or immune reactions and cell lysis, were observed in the tumours within one day of implantation. During development of the tumour, and during its regression after treatment with methylene dimethane sulphonate (MDMS), further changes were observed in the concentration of the species. These were dependent on the region of the tumour examined. In blood, development of the tumour produced an increase in ceruloplasmin and a decrease in iron-transferrin. An increase in spleen weight, as the tumour developed, was accompanied by a small decrease in the concentration of species with g-values of 6-0 and 4-3, which was reversed on regression of the treated tumour. The presence of metastases in the regional lymph nodes produced distinguishable changes in the ESR spectra.
利用电子自旋共振（ESR）光谱法检测雌性Wistar大鼠所携带的吉田瘤中顺磁性金属离子浓度的变化。还通过ESR对这些动物的血液、脾脏和淋巴结进行了检测。在植入后的一天内，观察到肿瘤中线粒体活性相关顺磁性物质的浓度降低，而那些被认为与炎症或免疫反应以及细胞裂解相关的物质浓度显著增加。在肿瘤的发展过程中以及在用亚甲基二甲烷磺酸盐（MDMS）治疗后肿瘤消退期间，观察到这些物质的浓度进一步变化。这些变化取决于所检测的肿瘤区域。在血液中，肿瘤的发展导致血浆铜蓝蛋白增加，转铁蛋白铁减少。随着肿瘤的发展，脾脏重量增加，同时g值为6.0和4.3的物质浓度略有下降，在治疗后的肿瘤消退时这种下降得到逆转。区域淋巴结中转移灶的存在使ESR光谱产生了可区分的变化。
