Macdonald Mara, Rodriguez Natalia M, Smith Renee, Hammond Paula T
Joint Harvard-MIT division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.
J Control Release. 2008 Nov 12;131(3):228-34. doi: 10.1016/j.jconrel.2008.07.032. Epub 2008 Jul 30.
Layer-by-layer (LbL) films have multiple features that make them attractive for drug delivery, including the potential to sequentially deliver growth factors from implantable medical devices or tissue engineering scaffolds. To date, however, characterization has been lacking for protein delivery from such films. Here, LbL polyelectrolyte films constructed with the model protein lysozyme and a hydrolytically degradable and biocompatible synthetic polycation are characterized. Milligram/cm(2) scale linear or power law release profiles can be achieved over 2 to 34 days, and control over loading and release are demonstrated through parameters such as tuning the degradability of the synthetic polycation, changing the number of layers used, or changing the polysaccharide polyanion. Functionality is maintained at nearly 100%, underscoring mild processing conditions apt to preserve fragile protein function. LbL films thus have promise as a tool for exploring protein modulation of the interaction between implanted surfaces and the cells they contact.
层层（LbL）膜具有多种特性，使其在药物递送方面具有吸引力，包括从可植入医疗设备或组织工程支架中依次递送生长因子的潜力。然而，迄今为止，此类膜蛋白递送的表征尚不完善。在此，对由模型蛋白溶菌酶和可水解降解且具有生物相容性的合成聚阳离子构建的LbL聚电解质膜进行了表征。在2至34天内可实现毫克/平方厘米级别的线性或幂律释放曲线，并且通过调整合成聚阳离子的降解性、改变使用的层数或改变多糖聚阴离子等参数来证明对负载和释放的控制。功能维持在近100%，这突出了适合保留脆弱蛋白质功能的温和加工条件。因此，LbL膜有望成为探索蛋白质对植入表面与其接触细胞之间相互作用进行调节的工具。
