McMillan A, Young H
Scottish Bacterial Sexually Transmitted Infections Reference Laboratory, Laboratory Medicine (Microbiology), 51 Little France Crescent, Edinburgh, Scotland, UK.
Int J STD AIDS. 2008 Sep;19(9):620-4. doi: 10.1258/ijsa.2008.008103.
The aim of the present study was to evaluate the use of various serological tests in the diagnosis of early syphilis. The Murex enzyme immunoassay (EIA) test was used for screening; the Venereal Diseases Research Laboratory (VDRL) test, the Treponema pallidum particle agglutination assay (TPPA) and the Mercia antitreponemal IgM EIA were used in all the patients with a positive screening test and in those with suspected syphilis or in known contacts. In 89 cases of primary syphilis, the Murex EIA screening test was positive in 67 (75%) patients, the Mercia IgM EIA in 80 (90%) cases, the VDRL in 60 (67%) cases and the TPPA in 85 (96%) cases. All the tests were positive in 68 patients with secondary syphilis. In 72 cases of early latent syphilis, the Murex EIA screening test was positive in 68 (94%) patients, the Mercia IgM EIA in 50 (69%) cases, the VDRL in 61 (85%) cases and the TPPA in 68 (94%) cases. The Mercia IgM EIA was the only test positive in four (6%) of these cases; these four patients were known contacts. Antibody titres in the VDRL and TPPA increased as the infection progressed.
本研究的目的是评估各种血清学检测在早期梅毒诊断中的应用。采用Murex酶免疫测定（EIA）试验进行筛查；对所有筛查试验阳性的患者、疑似梅毒患者或已知接触者，使用性病研究实验室（VDRL）试验、梅毒螺旋体颗粒凝集试验（TPPA）和Mercia抗梅毒螺旋体IgM EIA。在89例一期梅毒患者中，Murex EIA筛查试验67例（75%）阳性，Mercia IgM EIA 80例（90%）阳性，VDRL 60例（67%）阳性，TPPA 85例（96%）阳性。68例二期梅毒患者所有检测均为阳性。在72例早期潜伏梅毒患者中，Murex EIA筛查试验68例（94%）阳性，Mercia IgM EIA 50例（69%）阳性，VDRL 61例（85%）阳性，TPPA 68例（94%）阳性。Mercia IgM EIA是这些病例中4例（6%）唯一阳性的检测；这4例患者为已知接触者。随着感染进展，VDRL和TPPA中的抗体滴度升高。
