Termine J D, Conn K M
Calcif Tissue Res. 1976 Dec 22;22(2):149-57. doi: 10.1007/BF02010354.
Apatite formation from synthetic extracellular fluids is rate-limited both at the initial amorphous precursor deposition step and at the amorphous-crystalline transformation reaction. Nucleotide diphosphates and triphosphates and low molecular weight metabolites containing two attached ester phosphate groups all inhibited amorphous-crystalline conversion at concentrations of 10(-5) to 10(-6)M. Both native and synthetic polynucleotides as well as the phosphoproteins from rat dentin or egg yolk also inhibited crystal formation from amorphous calcium phosphate. In all cases, substantial amounts of inhibitor molecules were incorporated into the stabilized amorphous precipitates. Treatment of isolated, inhibitor-stabilized amorphous precipitates with hydrolytic enzymes such as alkaline phosphatase or papain reversed the inhibitory effect and permitted crystallization to proceed normally.
合成细胞外液中磷灰石的形成在初始无定形前体沉积步骤和无定形-结晶转变反应中均受到速率限制。核苷酸二磷酸和三磷酸以及含有两个连接酯磷酸基团的低分子量代谢物在浓度为10^(-5)至10^(-6)M时均抑制无定形-结晶转化。天然和合成多核苷酸以及大鼠牙本质或蛋黄中的磷蛋白也抑制了无定形磷酸钙的晶体形成。在所有情况下，大量抑制剂分子都掺入到稳定的无定形沉淀物中。用碱性磷酸酶或木瓜蛋白酶等水解酶处理分离出的、抑制剂稳定的无定形沉淀物可逆转抑制作用，并使结晶正常进行。
