Brighenti L, Puviani A C, Gavioli M E, Fabbri E, Ottolenghi C
Institute of General Physiology, University of Ferrara, Italy.
Gen Comp Endocrinol. 1991 Apr;82(1):131-9. doi: 10.1016/0016-6480(91)90304-o.
The simultaneous addition of epinephrine and salmon glucagon to catfish (Ictalurus melas) and trout (Salmo gairdneri) hepatocytes did not induce greater increases in glycogen phosphorylase a activity and in glucose release than those caused by epinephrine alone. The effects of epinephrine are greater than those of glucagon. Propranolol added to the hormonal pool blocked the epinephrine effects. In trout cells, epinephrine and glucagon-like peptide (GLP) had similar effects and when they were added simultaneously the stimulation of metabolic indices was higher compared to that obtained with either epinephrine or GLP. However, the effects were not additive. In the presence of epinephrine plus GLP the inhibitory effect of propranolol was not evident, due to the effect induced by GLP, on which propranolol was not effective. This may indicate that epinephrine masks the GLP effect. Results could mean that epinephrine and glucagon-family peptides act in catfish and trout hepatocytes through different receptors on the same pathway leading to glycogen phosphorylase a activation.
将肾上腺素和鲑鱼胰高血糖素同时添加到鲶鱼（黑真鮰）和虹鳟鱼（吉氏虹鳟）的肝细胞中，与单独使用肾上腺素相比，糖原磷酸化酶a活性和葡萄糖释放量的增加幅度并未更大。肾上腺素的作用大于胰高血糖素。添加到激素混合物中的普萘洛尔可阻断肾上腺素的作用。在虹鳟鱼细胞中，肾上腺素和胰高血糖素样肽（GLP）具有相似的作用，当它们同时添加时，与单独使用肾上腺素或GLP相比，代谢指标的刺激作用更高。然而，这些作用并非相加的。在存在肾上腺素加GLP的情况下，由于GLP诱导的作用，普萘洛尔的抑制作用不明显，而普萘洛尔对此无效。这可能表明肾上腺素掩盖了GLP的作用。结果可能意味着肾上腺素和胰高血糖素家族肽在鲶鱼和虹鳟鱼肝细胞中通过同一途径上的不同受体起作用，导致糖原磷酸化酶a激活。
