液相色谱法测定犊牛组织中的氯霉素：肌肉、肾脏和肝脏中的稳定性研究。

Liquid chromatographic determination of chloramphenicol in calf tissues: studies of stability in muscle, kidney, and liver.

作者信息

Sanders P, Guillot P, Dagorn M, Delmas J M

机构信息

Laboratoire des Medicaments Vétérinaires, Centre National d'Etudes Vétérinaires et Alimentaires, Ministère de l'Agriculture et de la Forêt, La Haute Marche-Javené, Fougères.

出版信息

J Assoc Off Anal Chem. 1991 May-Jun;74(3):483-6.

PMID:1874692

Abstract

A liquid chromatographic method has been developed for the measurement of chloramphenicol (CAP) in muscle, liver, and kidney. The mean recovery levels were 82.6, 75.3, and 79.2% in muscle, liver, and kidney, respectively. The method was repeatable and reproducible for CAP measurement in muscle, with a detection limit of 1 microgram/kg. Investigation of CAP stability in muscle, liver, and kidney showed that CAP stability in muscle was good at -20 degrees C; for spiked liver and kidney, degradation of CAP was observed, and the use of piperonyl butoxide (PB) for metabolism inhibition was recommended for recovery and linearity studies. However, PB was unnecessary for preservation of treated animal tissues if samples were cut into cubes and cooled at -20 degrees C, just after slaughter, pending analysis. With these limitations, CAP can be measured in liver and kidney.

摘要

已开发出一种液相色谱法用于测定肌肉、肝脏和肾脏中的氯霉素（CAP）。肌肉、肝脏和肾脏中的平均回收率分别为82.6%、75.3%和79.2%。该方法在肌肉中测量CAP具有可重复性和再现性，检测限为1微克/千克。对肌肉、肝脏和肾脏中CAP稳定性的研究表明，肌肉中CAP在-20℃时稳定性良好；对于加标肝脏和肾脏，观察到CAP有降解现象，建议在回收率和线性研究中使用胡椒基丁醚（PB）抑制代谢。然而，如果在屠宰后立即将样品切成小块并在-20℃下冷却，以待分析，则PB对于处理过的动物组织的保存并非必需。尽管有这些限制，仍可在肝脏和肾脏中测量CAP。