Azevedo A L S, Nascimento C S, Steinberg R S, Carvalho M R S, Peixoto M G C D, Teodoro R L, Verneque R S, Guimarães S E F, Machado M A
Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
Genet Mol Res. 2008 Jul 15;7(3):623-30. doi: 10.4238/vol7-3gmr428.
Frequencies of kappa-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Gyr cows, Guzerat sires, Guzerat cows, Nellore sires, and Gyr x Holstein crossbreds) by means of polymerase chain reaction-restriction fragment length polymorphism analysis using two independent restriction nucleases (Hinf I and HaeIII). The genotyping of kappa-casein alleles (A and B) is of practical importance, since the B allele is found to correlate with commercially valuable parameters of cheese yielding efficiency. The frequencies of the B allele of kappa-casein among breeds ranged from 0.01 to 0.30. The Sindhi breed had the highest frequency for the B allele (0.30), while the frequencies of this allele in other breeds ranged from 0.01 to 0.18. A wide variation in the B allele frequency among B. indicus breeds was found suggesting that molecular selection for animals carrying the B allele could impact breeding programs for dairy production.
通过使用两种独立的限制性核酸酶（Hinf I和HaeIII）进行聚合酶链反应-限制性片段长度多态性分析，测定了来自巴西印度瘤牛遗传群体（信德牛、吉尔公牛、吉尔母牛、古扎拉特公牛、古扎拉特母牛、内洛尔公牛和吉尔×荷斯坦杂交种）的1316头动物中κ-酪蛋白基因等位基因的频率。κ-酪蛋白等位基因（A和B）的基因分型具有实际重要性，因为发现B等位基因与奶酪生产效率的商业价值参数相关。κ-酪蛋白B等位基因在各品种中的频率范围为0.01至0.30。信德品种的B等位基因频率最高（0.30），而该等位基因在其他品种中的频率范围为0.01至0.18。发现印度瘤牛品种间B等位基因频率存在广泛差异，这表明对携带B等位基因的动物进行分子选择可能会影响奶牛生产的育种计划。
