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法国阿尔卑斯山羊、努比亚山羊、克里奥尔山羊奶与荷斯坦奶牛牛奶蛋白质组成的比较分析:对婴儿早期营养的影响

Comparative Analysis of the Protein Composition of Goat Milk from French Alpine, Nubian, and Creole Breeds and Holstein Friesian Cow Milk: Implications for Early Infant Nutrition.

作者信息

Muñoz-Salinas Florencia, Andrade-Montemayor Héctor Mario, De la Torre-Carbot Karina, Duarte-Vázquez Miguel Ángel, Silva-Jarquin Juan Carlos

机构信息

Facultad de Ingeniería, Campus Amazcala, Universidad Autónoma de Querétaro, El Marqués, CP 76265, Mexico.

Facultad de Ciencias Naturales, Campus Juriquilla, Universidad Autónoma de Querétaro, Juriquilla, CP 76230, Mexico.

出版信息

Animals (Basel). 2022 Aug 30;12(17):2236. doi: 10.3390/ani12172236.

Abstract

Of the diversity of proteins and high digestibility, goat milk will be a food of significant value for infant nutrition. The genetic polymorphisms of milk proteins play an essential role in the different degrees of allergic reactions. This work aimed to identify the proteins and peptides in the composition of goat milk and compare them to those in cow's milk. The work was performed with goats French Alpine, Nubian, and Creole breeds and Holstein Friesian milking cows at the Universidad Autónoma de Querétaro, Amazcala. We investigated the relative abundance of goat and cow milk protein fractions by SDS-PAGE resolution and the densitometric analysis of gels. The protein alfa-casein was (17.67 ± 0.46) for Creole, (19.18 ± 0.88) French Alpine, (17.35 ± 0.49) Nubian, and (35.92 ± 1.96) Holstein cows. The relative abundance obtained from alfa-casein was statistically different between goats and cows, and this protein was vital because it is a protein related to allergies. On the other hand, the amino acid in position 67 of the beta-casein from three goat breeds is a Proline, so it is assumed that the beta-casein variant of goat milk is an A2-type. The latter has excellent relevance for infant nutrition and differs from cow milk.

摘要

鉴于蛋白质的多样性和高消化率,山羊奶将成为婴儿营养中具有重要价值的食物。乳蛋白的基因多态性在不同程度的过敏反应中起着至关重要的作用。这项工作旨在鉴定山羊奶成分中的蛋白质和肽,并将它们与牛奶中的进行比较。研究在克雷塔罗自治大学阿马斯卡拉分校使用法国阿尔卑斯山羊、努比亚山羊和克里奥尔山羊品种以及荷斯坦奶牛进行。我们通过SDS-PAGE分离和凝胶密度分析研究了山羊奶和牛奶蛋白组分的相对丰度。克里奥尔山羊的α-酪蛋白为(17.67±0.46),法国阿尔卑斯山羊为(19.18±0.88),努比亚山羊为(17.35±0.49),荷斯坦奶牛为(35.92±1.96)。山羊和奶牛的α-酪蛋白相对丰度在统计学上存在差异,这种蛋白质很重要,因为它是一种与过敏相关的蛋白质。另一方面,三个山羊品种的β-酪蛋白第67位的氨基酸是脯氨酸,因此可以认为山羊奶的β-酪蛋白变体是A2型。后者对婴儿营养具有重要意义,且与牛奶不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/392d/9454708/3cd79266aeeb/animals-12-02236-g001.jpg

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