Maderazo E G, Woronick C L, Hickingbotham N, Jacobs L, Bhagavan H N
Medical Research Laboratory, Department of Medicine, Hartford Hospital, CT 06115.
J Trauma. 1991 Aug;31(8):1142-50.
Studies in patients with serious trauma indicate that the observed neutrophil (PMN) locomotory dysfunction is partly the result of auto-oxidation as shown by evidence of preactivation, diminished reducing capacity, and low serum and cellular ascorbic acid and alpha-tocopherol. To investigate whether replacement of the antioxidant vitamins ascorbic acid and alpha-tocopherol can improve the PMN locomotory defect, ascorbic acid, alpha-tocopherol, ascorbic acid and alpha-tocopherol, or placebo was administered to a total of 46 victims of blunt trauma. PMN locomotion was quantitated using a micropore filter assay. Locomotion data were analyzed by repeated measures analysis with a split plot design and data for days 2-6 after injury were compared. Compared with placebo, the antioxidants improved PMN locomotion. The mean differences in distance migrated (treated minus placebo) were ascorbic acid and alpha-tocopherol = 11.3 +/- 3.0 microns (one-tailed p = 0.001) (mean +/- SE); ascorbic acid = 4.7 +/- 3.4 microns (p = 0.19); and alpha-tocopherol = 3.3 +/- 2.9 microns (p = 0.27). Although both antioxidants given together produced the best results, a plot of the 95% confidence intervals indicates that ascorbic acid and alpha-tocopherol, either given alone, were also better than placebo. We conclude that antioxidant replacement therapy significantly improves the PMN locomotory abnormality in blunt trauma.
对严重创伤患者的研究表明，观察到的中性粒细胞（PMN）运动功能障碍部分是自氧化的结果，这表现为预激活、还原能力下降、血清和细胞中抗坏血酸及α-生育酚水平低的证据。为了研究补充抗氧化维生素抗坏血酸和α-生育酚是否能改善PMN运动缺陷，对总共46名钝性创伤受害者给予抗坏血酸、α-生育酚、抗坏血酸和α-生育酚或安慰剂。使用微孔滤膜试验对PMN运动进行定量。采用裂区设计的重复测量分析对运动数据进行分析，并比较受伤后第2至6天的数据。与安慰剂相比，抗氧化剂改善了PMN运动。迁移距离的平均差异（治疗组减去安慰剂组）为：抗坏血酸和α-生育酚=11.3±3.0微米（单尾p=0.001）（平均值±标准误）；抗坏血酸=4.7±3.4微米（p=0.19）；α-生育酚=3.3±2.9微米（p=0.27）。虽然同时给予两种抗氧化剂产生了最佳效果，但95%置信区间图表明，单独给予抗坏血酸和α-生育酚也比安慰剂更好。我们得出结论，抗氧化剂替代疗法能显著改善钝性创伤中PMN的运动异常。
