Maderazo E G, Woronick C L, Hickingbotham N, Mercier E, Jacobs L, Bhagavan H
Medical Research Laboratory, Hartford Hospital, CT 06115.
Crit Care Med. 1990 Feb;18(2):141-7. doi: 10.1097/00003246-199002000-00003.
Previous studies in victims of blunt injury suggest that the observed neutrophil (PMN) locomotory dysfunction is, in part, due to autoxidation. To further clarify the occurrence and significance of autoxidation, we studied changes in levels of glutathione in PMN and of ascorbic acid and alpha-tocopherol in serum and blood cells of postsurgical and blunt trauma patients. Levels of total, reduced, and oxidized glutathione in PMN from trauma patients were similar to normal controls. Serum and cellular ascorbic acid and alpha-tocopherol levels dropped significantly after injury and remained below normal control levels during the 7 to 8-day study period. Low serum alpha-tocopherol was partially explainable on the basis of changes in serum lipids. When serum samples of trauma patients were thawed unprotected without pyrogallol, there was significant loss of recoverable alpha-tocopherol, whereas no significant losses occurred with unprotected thawed normal sera. Less total reducing capacity was observed in PMN of trauma patients compared with normal controls. These findings indicate that synthesis and regeneration capacity of glutathione are intact but that the levels of the consumable antioxidants, ascorbic acid, and alpha-tocopherol are compromised after injury. These results add further support to the hypothesis that autoxidation occurs in trauma.
以往对钝性损伤受害者的研究表明，观察到的中性粒细胞（PMN）运动功能障碍部分归因于自氧化作用。为了进一步阐明自氧化作用的发生情况及意义，我们研究了手术患者和钝性创伤患者的中性粒细胞中谷胱甘肽水平以及血清和血细胞中抗坏血酸和α-生育酚水平的变化。创伤患者中性粒细胞中总谷胱甘肽、还原型谷胱甘肽和氧化型谷胱甘肽的水平与正常对照组相似。损伤后血清和细胞中的抗坏血酸及α-生育酚水平显著下降，并在7至8天的研究期内一直低于正常对照组水平。血清α-生育酚水平降低部分可根据血清脂质的变化来解释。当创伤患者的血清样本在没有焦性没食子酸保护的情况下解冻时，可回收的α-生育酚显著损失，而未受保护解冻的正常血清则没有显著损失。与正常对照组相比，创伤患者中性粒细胞的总还原能力较低。这些发现表明，谷胱甘肽的合成和再生能力是完整的，但损伤后可消耗的抗氧化剂抗坏血酸和α-生育酚的水平受到了损害。这些结果进一步支持了创伤中发生自氧化作用的假说。
