Pinner S, Sahai E
Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, LONDON WC2A 3PX, United Kingdom.
J Microsc. 2008 Sep;231(3):441-5. doi: 10.1111/j.1365-2818.2008.02056.x.
The availability of multi-photon intravital microscopy has recently allowed researchers to start to visualise the dynamic behaviour of cancer cells in vivo. This imaging has revealed that many cancer cells ranging from carcinoma to melanoma move in an amoeboid manner in order to invade surrounding tissue and escape from the primary tumour. This mode on cell motility is extremely rapid and does not require the activity of proteases to degrade the extra-cellular matrix (ECM). This review details the techniques that are available to study cell motility in vivo and discusses the current knowledge about the mechanisms of amoeboid cell motility.
多光子活体显微镜的出现，使得研究人员最近能够开始在体内观察癌细胞的动态行为。这种成像技术揭示，从癌细胞到黑色素瘤细胞等许多癌细胞，都会以变形虫样的方式移动，从而侵入周围组织并逃离原发肿瘤。这种细胞运动模式极其迅速，且不需要蛋白酶的活性来降解细胞外基质（ECM）。这篇综述详细介绍了用于研究体内细胞运动的现有技术，并讨论了目前关于变形虫样细胞运动机制的知识。
