ExCel Matrix Biological Devices Pvt. Ltd. 12-5-149/16-2, Vijayapuri (Opp. NIN), South Lalaguda, Hyderabad, India.
Biotechnol Adv. 2010 Nov-Dec;28(6):764-9. doi: 10.1016/j.biotechadv.2010.06.002. Epub 2010 Jun 18.
Tissue regeneration and cell therapy have an enormous potential in healthcare through the creation of artificial human tissues and organs. The possibility of producing functional replica of tissues and organs can offer a common, solitary solution for various kinds of inflictions. It can also provide an ultimate test model for drug discovery. There exists convincing evidence that if cells are cultured in extra-cellular matrix (ECM) mimicking 3D scaffolds infused with tissue-specific biochemical cues they grow and differentiate to express functionality. However, comprehensive understanding of ECM and its dynamic relation with the growing cells is vital for creating functional tissue models ex vivo. Different medical and non-medical groups all over the world are working towards achieving affordable, user friendly and technically viable solutions for improving our understanding of Cell-ECM dynamics for tissue engineering (TE). Successful TE, an ambitious goal that includes tissue neogenesis in vitro and functional tissue mending (regenerative medicine) in vivo, however involves innumerable challenges. Present review discusses some of the major technical hurdles that hinder the pace of progress in tissue regeneration/engineering (TE).
组织再生和细胞疗法通过创建人工人体组织和器官，在医疗保健方面具有巨大的潜力。生产具有功能性的组织和器官复制品的可能性为各种疾病提供了一种通用的单一解决方案。它还可以为药物发现提供最终的测试模型。有令人信服的证据表明，如果细胞在细胞外基质（ECM）中培养，这些 ECM 模仿 3D 支架，其中注入了组织特异性的生化线索，那么它们会生长和分化，以表达功能。然而，为了在体外创建功能性组织模型，全面了解 ECM 及其与生长细胞的动态关系至关重要。世界各地的不同医学和非医学团体都在努力实现负担得起、用户友好和技术可行的解决方案，以提高我们对细胞-ECM 动力学在组织工程（TE）中的理解。然而，成功的 TE 是一个雄心勃勃的目标，包括体外组织新生和体内功能性组织修复（再生医学），涉及无数的挑战。本综述讨论了一些阻碍组织再生/工程（TE）进展的主要技术障碍。
