测定脑心肌炎病毒、脊髓灰质炎病毒、鼻病毒、RAV - 61和菜豆金色花叶病毒的病毒粒子RNA以及小鼠珠蛋白mRNA 3'端多聚腺苷酸的长度分布。
Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.
作者信息
Ahlquist P, Kaesberg P
出版信息
Nucleic Acids Res. 1979 Nov 10;7(5):1195-204. doi: 10.1093/nar/7.5.1195.
Abstract
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.
摘要
通过用T4 RNA连接酶对RNA进行3'末端32P标记、用核糖核酸酶T1和A消化以及使用凝胶测序方法，可以对RNA群体中3'末端多聚（A）的长度谱进行快速、详细和准确的分析。本文展示了脑心肌炎病毒、脊髓灰质炎病毒、鼻病毒、RAV - 61和豇豆花叶病毒病毒粒子RNA以及小鼠珠蛋白mRNA的3'末端多聚（A）的长度分布。
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