Lao Yang-Jun, Xu Tao-Tao, Jin Hong-Ting, Ruan Hong-Feng, Wang Ji-Tao, Zhou Li, Wang Ping-Er, Wang Jian, Ying Jun, Zhang Yuan-Bin, Luo Cheng, Fu Fang-da, Tong Pei-Jian, Xiao Lu-Wei, Wu Cheng-Liang
Institute of Orthopaedics and Traumatology, Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Zhejiang, China.
Department of Orthopaedics, Tongde Hospital, Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Zhejiang, China.
Orthop Surg. 2018 Feb;10(1):56-63. doi: 10.1111/os.12365. Epub 2018 Feb 12.
To investigate the effect of accumulated spinal axial biomechanical loading on mice lumbar disc and the feasibility of applying this method to establish a mice intervertebral disc degeneration model using a custom-made hot plate cage. In previous studies, we observed that the motion pattern of mice was greatly similar to that of humans when they were standing and jumping on their lower limbs. There is little data to demonstrate whether or not accumulated spinal axial biomechanical loading could induce intervertebral disc degeneration in vivo.
Twenty-four 0-week-old mice were randomly divided into model 1-month and 3-month groups, and control 1-month and 3-month groups (n = 6 per group). The model groups was transferred into the custom-made hot plate cage three times per day for modeling. The control group was kept in a regular cage. The intervertebral disc samples of the L -L were harvested for histologic, molecular, and immunohistochemical studies after modeling for 1 and 3 months.
Accumulated spinal axial biomechanical loading affects the histologic, molecular, and immunohistochemical changes of mice L L intervertebral discs. Decreased height of disc and endplate, fissures of annulus fibrosus, and ossification of cartilage endplate were found in morphological studies. Immunohistochemical studies of the protein level showed a similar expression of type II collagen at 1 month, but a slightly decreased expression at 3 months, and an increased expression level of type X collagen and matrix metalloproteinase 13 (MMP13). Molecular studies showed that ColIIa1 and aggrecan mRNA expression levels were slightly increased at 1 month (P > 0.05), but then decreased slightly (P > 0.05). ColXa1, ADAMTS-5, and MMP-13 expression levels werer increased both at 1 and 3 months (P < 0.05). In addition, increased expression of Runx2 was observed.
Accumulated spinal axial loading provided by a custom-made hot plate accelerated mice lumbar disc and especially endplate degeneration. However, this method requires further development to establish a lumbar disc degeneration model.
探讨累积性脊柱轴向生物力学负荷对小鼠腰椎间盘的影响，以及使用定制热板笼应用该方法建立小鼠椎间盘退变模型的可行性。在先前的研究中，我们观察到小鼠在下肢站立和跳跃时的运动模式与人类非常相似。几乎没有数据表明累积性脊柱轴向生物力学负荷是否能在体内诱导椎间盘退变。
将24只0周龄小鼠随机分为模型1个月组和3个月组，以及对照1个月组和3个月组（每组n = 6）。模型组每天转移到定制热板笼中三次进行建模。对照组饲养在常规笼子里。建模1个月和3个月后，采集L - L节段的椎间盘样本进行组织学、分子学和免疫组织化学研究。
累积性脊柱轴向生物力学负荷影响小鼠L L节段椎间盘的组织学、分子学和免疫组织化学变化。形态学研究发现椎间盘和终板高度降低、纤维环裂隙以及软骨终板骨化。蛋白质水平的免疫组织化学研究显示，II型胶原蛋白在1个月时表达相似，但在3个月时略有下降，而X型胶原蛋白和基质金属蛋白酶13（MMP13）的表达水平增加。分子学研究表明，ColIIa1和聚集蛋白聚糖mRNA表达水平在1个月时略有增加（P > 0.05），但随后略有下降（P > 0.05）。ColXa1、ADAMTS - 5和MMP - 13表达水平在1个月和3个月时均升高（P < 0.05）。此外，观察到Runx2表达增加。
定制热板提供的累积性脊柱轴向负荷加速了小鼠腰椎间盘尤其是终板的退变。然而，该方法需要进一步改进以建立腰椎间盘退变模型。
