Department of Orthopaedics and Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
Spine (Phila Pa 1976). 2011 Jan 1;36(1):E14-9. doi: 10.1097/BRS.0b013e3181d2dec2.
An in vivo study of the cervical intervertebral discs (IVDs) response to upright posture was performed using an amputated bipedal rat model.
To investigate the effects of upright posture on IVDs of rat cervical spine.
The distinct arrangement of human neck muscle from that of cat and rhesus indicated that in the evolution process, upright posture might have affected cervical spine of human ancestors. However, the effects of upright posture on cervical spine have not been assessed.
Forty-one-month-old rats were randomly divided into 5-month-control, 5-month-surgery, 7-month-control, and 7-month surgery group (n = 10 per group). Both forelimbs of 2 surgery group rats were amputated, and those rats were then induced to be upright in the custom-made cages. Two control group rats were kept in regular cages. These rats were respectively killed at the fifth and seventh month after surgery and the IVD samples of lumbar spine were harvested for histologic and immunohistochemical studies. Total RNA isolated from these samples were used for real-time polymerase chain reaction of type II collagen (Col2a1), type X collagen, matrix metalloproteinase 13 (MMP-13), MMP-3, aggre-can, and aggrecanase-2 (ADAMTS-5).
Upright posture affects histologic changes of the cervical IVDs such as fissures of anulus fibrosus and decreased height of disc, decreased protein level of Col2a1 at nucleus pulposus and anulus fibrosus, up-regulated MMP-13, MMP-3, ADAMTS-5, and type X collagen mRNA expression, and downregulated mRNA expression of Col2a1 and aggrecan.
Upright stance accelerates cervical disc degeneration in rats.
采用双后肢截除大鼠模型进行一项颈椎间盘（IVD）对直立姿势反应的体内研究。
研究直立姿势对大鼠颈椎间盘的影响。
人类颈部肌肉的排列方式与猫和恒河猴明显不同，这表明在进化过程中，直立姿势可能影响了人类祖先的颈椎。然而，直立姿势对颈椎的影响尚未得到评估。
41 月龄大鼠随机分为 5 个月对照组、5 个月手术组、7 个月对照组和 7 个月手术组（每组 10 只）。2 个手术组大鼠的双前肢被截除，然后将这些大鼠置于定制笼中诱导直立。2 个对照组大鼠则被饲养在常规笼中。术后第 5 个月和第 7 个月时，分别处死这两组大鼠，并取其腰椎 IVD 样本进行组织学和免疫组织化学研究。从这些样本中提取总 RNA，用于 II 型胶原（Col2a1）、X 型胶原、基质金属蛋白酶 13（MMP-13）、MMP-3、聚集蛋白聚糖和聚集蛋白聚糖酶-2（ADAMTS-5）的实时聚合酶链反应。
直立姿势影响颈椎 IVD 的组织学变化，如纤维环裂隙和椎间盘高度降低，核髓和纤维环中 Col2a1 蛋白水平降低，MMP-13、MMP-3、ADAMTS-5 和 X 型胶原 mRNA 表达上调，而 Col2a1 和聚集蛋白聚糖的 mRNA 表达下调。
直立姿势加速了大鼠颈椎间盘的退变。
