Click chemistry for high-density biofunctionalization of mesoporous silica.

The applicability of click chemistry for high-density functionalization of mesoporous silica is demonstrated. The mild conditions of the copper(I)-catalyzed Huisgen reaction allow for a surface functionalization with intact biomolecules. The high covalent enzyme functionalization density under simultaneous retention of enzyme activity and the absence of leaching demonstrate the promising potential of this approach.