Schlossbauer Axel, Schaffert David, Kecht Johann, Wagner Ernst, Bein Thomas
Department of Chemistry and Biochemistry, Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstr. 5-13 (E), 81377 Munich, Germany.
J Am Chem Soc. 2008 Sep 24;130(38):12558-9. doi: 10.1021/ja803018w. Epub 2008 Aug 29.
The applicability of click chemistry for high-density functionalization of mesoporous silica is demonstrated. The mild conditions of the copper(I)-catalyzed Huisgen reaction allow for a surface functionalization with intact biomolecules. The high covalent enzyme functionalization density under simultaneous retention of enzyme activity and the absence of leaching demonstrate the promising potential of this approach.
证明了点击化学在介孔二氧化硅高密度功能化中的适用性。铜（I）催化的惠斯根反应的温和条件允许用完整的生物分子进行表面功能化。在同时保留酶活性且无浸出的情况下，高共价酶功能化密度证明了该方法具有广阔的应用前景。
