Jers Carsten, Soufi Boumediene, Grangeasse Christophe, Deutscher Josef, Mijakovic Ivan
Technical University of Denmark, Biosys, Center for Microbial Biotechnology, DK-2800 Lyngby, Denmark.
Expert Rev Proteomics. 2008 Aug;5(4):619-27. doi: 10.1586/14789450.5.4.619.
Bacteria use protein phosphorylation to regulate all kinds of physiological processes. Protein phosphorylation plays a role in several key steps of the infection process of bacterial pathogens, such as adhesion to the host, triggering and regulation of pathogenic functions as well as biochemical warfare; scrambling the host signaling cascades and impairing its defense mechanisms. Recent phosphoproteomic studies indicate that the bacterial protein phosphorylation networks could be more complex than initially expected, comprising promiscuous kinases that regulate several distinct cellular functions by phosphorylating different protein substrates. Recent advances in protein labeling with stable isotopes in the field of quantitative mass spectrometry phosphoproteomics will enable us to chart the global phosphorylation networks and to understand the implication of protein phosphorylation in cellular regulation on the systems scale. For the study of bacterial pathogens, in particular, this research avenue will enable us to dissect phosphorylation-related events during different stages of infection and stimulate our efforts to find inhibitors for key kinases and phosphatases implicated therein.
细菌利用蛋白质磷酸化来调节各种生理过程。蛋白质磷酸化在细菌病原体感染过程的几个关键步骤中发挥作用，例如与宿主的粘附、致病功能的触发和调节以及生化战；扰乱宿主信号级联反应并损害其防御机制。最近的磷酸化蛋白质组学研究表明，细菌蛋白质磷酸化网络可能比最初预期的更为复杂，其中包括通过磷酸化不同蛋白质底物来调节多种不同细胞功能的混杂激酶。定量质谱磷酸化蛋白质组学领域中稳定同位素蛋白质标记技术的最新进展，将使我们能够绘制全局磷酸化网络，并从系统层面理解蛋白质磷酸化在细胞调节中的意义。特别是对于细菌病原体的研究，这条研究途径将使我们能够剖析感染不同阶段与磷酸化相关的事件，并激励我们努力寻找其中涉及的关键激酶和磷酸酶的抑制剂。
