Ko Kwan Soo, Kim Yeon-Sook, Lee Mi Young, Shin So Young, Jung Dong Sik, Peck Kyong Ran, Song Jae-Hoon
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2164-8. doi: 10.1099/ijs.0.65534-0.
A Gram-positive, rod-shaped bacterium, designated strain LBY(T), was isolated from the blood of a Korean patient. The organism could not be identified based on conventional clinical microbiological methods. 16S rRNA gene sequencing and phylogenetic analysis revealed that it belonged to the genus Paenibacillus, but was distinct from recognized Paenibacillus species. Strain LBY(T) was related most closely to the type strains of Paenibacillus macerans and Paenibacillus popilliae, with 16S rRNA gene sequence similarities of 96.2 and 95.4%, respectively. The major fatty acids of strain LBY(T) were anteiso-C(15:0) (43.8%), C(16:0) (10.4%), iso-C(16:0) (10.3%), iso-C(15:0) (9.2%) and anteiso-C(17:0) (8.3%). On the basis of biochemical characteristics, fatty acid composition and comparative 16S rRNA gene sequence analysis, strain LBY(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus konsidensis sp. nov. is proposed. The type strain is LBY(T) (=ABB-ID-KSY9(T)=KCTC 13165(T)=JCM 14798(T)).
从一名韩国患者血液中分离出一株革兰氏阳性杆状细菌，命名为LBY(T)菌株。基于传统临床微生物学方法无法鉴定该菌株。16S rRNA基因测序和系统发育分析表明，它属于类芽孢杆菌属，但与已知的类芽孢杆菌物种不同。LBY(T)菌株与浸麻类芽孢杆菌和日本金龟子类芽孢杆菌的模式菌株关系最为密切，16S rRNA基因序列相似性分别为96.2%和95.4%。LBY(T)菌株的主要脂肪酸为anteiso-C(15:0)(43.8%)、C(16:0)(10.4%)、iso-C(16:0)(10.3%)、iso-C(15:0)(9.2%)和anteiso-C(17:0)(8.3%)。基于生化特性、脂肪酸组成和16S rRNA基因序列比较分析，LBY(T)菌株被认为代表类芽孢杆菌属的一个新物种，为此提出新名称康氏类芽孢杆菌（Paenibacillus konsidensis sp. nov.）。模式菌株为LBY(T)(=ABB-ID-KSY9(T)=KCTC 13165(T)=JCM 14798(T))。
