Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea.
Curr Microbiol. 2020 Mar;77(3):509-515. doi: 10.1007/s00284-019-01843-0. Epub 2019 Dec 12.
A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20, was isolated from the human subgingival dental plaque of a gingivitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus. BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593 (97.7% similarity) and Paenibacillus macerans ATCC 8244 (97.6% similarity). Draft genome of strain ChDC PVNT-B20 was composed of 8,112,407 bp. The DNA G+C content of the strain was 51.3 mol%. Average nucleotide identity values between strain ChDC PVNT-B20 and P. faecis DSM 23593 or P. macerans ATCC 8244 were 75.71% and 91.5%, respectively. Genome-to-genome distance values between strain ChDC PVNT-B20 and P. faecis DSM 23593 or P. macerans ATCC 8244 were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively. Major cellular fatty acids of strain ChDC PVNT-B20 were anteiso-C (43.4%), C (16.6%), iso-C (14.4%), and anteiso-C (12.4%). The sole respiratory quinone of the strain was menaqinone-7. Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL). Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3). Based on these results, strain ChDC PVNT-B20 is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp. nov. is proposed. Type strain is ChDC PVNT-B20 (= KCOM 3021 = JCM 33462).
一株革兰氏阴性、兼性厌氧、产芽孢、运动、杆状细菌，菌株 ChDC PVNT-B20，从牙龈炎病变的人类龈下牙菌斑中分离得到。基于 16S 核糖体 RNA 基因（16S rDNA）的系统发育分析表明，该菌株属于类芽孢杆菌属。菌株 16S rDNA 的 BLAST 分析显示与粪芽孢杆菌 DSM 23593（97.7%相似性）和粘质沙雷氏菌 ATCC 8244（97.6%相似性）具有很高的同源性。菌株 ChDC PVNT-B20 的基因组草图由 8112407 bp 组成。该菌株的 DNA G+C 含量为 51.3 mol%。菌株 ChDC PVNT-B20 与粪芽孢杆菌 DSM 23593 或粘质沙雷氏菌 ATCC 8244 的平均核苷酸同一性值分别为 75.71%和 91.5%。菌株 ChDC PVNT-B20 与粪芽孢杆菌 DSM 23593 或粘质沙雷氏菌 ATCC 8244 的基因组到基因组距离值分别为 21.6%（19.3-24.0%）和 44.9%（42.3-47.4%）。菌株 ChDC PVNT-B20 的主要细胞脂肪酸为anteiso-C（43.4%）、C（16.6%）、iso-C（14.4%）和 anteiso-C（12.4%）。该菌株的唯一呼吸醌为menaquinone-7。该菌株的主要极性脂为磷脂酰甘油（PG）、双磷脂酰甘油（DPG）和一种未鉴定的糖脂（GL）。次要极性脂为一种未鉴定的胺脂（AL）、一种未鉴定的磷脂（PL）和三种未鉴定的脂（L1-L3）。基于这些结果，菌株 ChDC PVNT-B20 被认为代表类芽孢杆菌属的一个新种，命名为口腔类芽孢杆菌（Paenibacillus oralis）sp. nov.。模式菌株为 ChDC PVNT-B20（=KCOM 3021=JCM 33462）。
