Huang Wei, Kanehira Kazunori, Drew Sally, Pier Thomas
Immunohistochemistry Laboratory, Department of Pathology and Laboratory Medicine, University of Wisconsin, 2500 Overlook Terrace, Madison WI 53792, USA.
Appl Immunohistochem Mol Morphol. 2009 Jan;17(1):12-7. doi: 10.1097/PAI.0b013e318173e79d.
Differentiating oncocytoma from its renal cell carcinoma (RCC) mimics, particularly chromophobe RCC, can be difficult, especially when limited tissue is available for evaluation. This study presents a panel of markers that are readily available, easy to use, and useful for differential diagnoses of renal tumors.
A renal cell neoplasm tissue microarray was constructed including oncocytoma (n=30), chromophobe RCC (n=18), conventional RCC (n=64), papillary RCC (n=50), and benign renal tissues (n=31). CK7, CD10, epithelial membrane antigen, renal cell carcinoma marker (RCCma), vimentin, and endogenous avidin-binding activity (EABA) were studied. An Automated Cellular Imaging System, was used to quantify the staining intensity.
EABA was positive in 97% of oncocytoma, 26% of conventional RCC and 35% of papillary RCC with granular/eosinophilic (G/E) features and 6% of chromophobe RCC. EABA was negative in RCC without G/E features. Vimentin and RCCma were positive in most RCC with G/E features (conventional, 78% and 71%; and papillary, 85% and 76%, respectively), and negative in oncocytoma. Vimentin was also negative in chromophobe RCC. CK7 was positive in up to 81% of papillary RCC and 63% of chromophobe RCC, and essentially negative in conventional RCC and oncocytoma.
EABA is an excellent marker for oncocytoma, which can be useful in differentiating oncocytoma from chromophobe RCC. A panel of EABA, vimentin, and RCCma markers can be useful in discerning oncocytoma from RCC with G/E features. Vimentin can be useful in discriminating chromophobe RCC from papillary or conventional RCCs.
将嗜酸细胞瘤与其肾细胞癌（RCC）的模仿物，尤其是嫌色性RCC区分开来可能很困难，特别是当用于评估的组织有限时。本研究提出了一组易于获得、易于使用且有助于肾肿瘤鉴别诊断的标志物。
构建了一个肾细胞肿瘤组织微阵列，包括嗜酸细胞瘤（n = 30）、嫌色性RCC（n = 18）、传统RCC（n = 64）、乳头状RCC（n = 50）和良性肾组织（n = 31）。研究了细胞角蛋白7（CK7）、CD10、上皮膜抗原、肾细胞癌标志物（RCCma）、波形蛋白和内源性抗生物素蛋白结合活性（EABA）。使用自动细胞成像系统对染色强度进行定量。
EABA在97%的嗜酸细胞瘤、26%的具有颗粒/嗜酸性（G/E）特征的传统RCC、35%的具有G/E特征的乳头状RCC和6%的嫌色性RCC中呈阳性。EABA在无G/E特征的RCC中呈阴性。波形蛋白和RCCma在大多数具有G/E特征的RCC中呈阳性（传统RCC分别为78%和71%；乳头状RCC分别为85%和76%），而在嗜酸细胞瘤中呈阴性。波形蛋白在嫌色性RCC中也呈阴性。CK7在高达81%的乳头状RCC和63%的嫌色性RCC中呈阳性，而在传统RCC和嗜酸细胞瘤中基本呈阴性。
EABA是嗜酸细胞瘤的一种优秀标志物，可用于将嗜酸细胞瘤与嫌色性RCC区分开来。一组EABA、波形蛋白和RCCma标志物可用于将嗜酸细胞瘤与具有G/E特征的RCC区分开来。波形蛋白可用于将嫌色性RCC与乳头状或传统RCC区分开来。
