Merritt Judith H, Kadouri Daniel E, O'Toole George A
Dartmouth Medical School, Hanover, New Hampshire, USA.
Curr Protoc Microbiol. 2005 Jul;Chapter 1:Unit 1B.1. doi: 10.1002/9780471729259.mc01b01s00.
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.
许多细菌能够以称为生物膜的表面附着聚集体形式存在。本单元介绍了几种研究这些群落的方法。这里重点介绍静态生物膜系统，该系统对于研究生物膜形成的早期阶段特别有用，包括对表面的初始附着和微菌落形成。此外，所介绍的大多数技术都很容易适用于在各种条件下研究生物膜，并且适用于小规模或相对大规模的研究。与涉及连续流动系统的测定不同，这里描述的静态生物膜测定所需的专用设备很少，并且执行起来相对简单。此外，这些静态生物膜系统允许通过多种读数分析生物膜形成，包括活细胞显微镜检查、染色细菌的宏观可视化和活力计数。单独使用或组合使用这些测定方法，为生物膜研究提供了有用的手段。
